| 致病性大肠杆菌苗用菌株的原生质体融合的研究 |
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| 引用本文： | 苟想珍,党岩,孙春香,马志宏,杨增歧,王晶珏,陈德坤.致病性大肠杆菌苗用菌株的原生质体融合的研究[J].畜牧兽医杂志,2010,29(5):36-39. |
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| 作者姓名： | 苟想珍 党岩 孙春香 马志宏 杨增歧 王晶珏 陈德坤 |
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| 作者单位： | 1. 甘肃省畜牧兽医研究所,甘肃,平凉,744000
2. 西北农林科技大学 |
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| 基金项目： | 甘肃省技术研究与开发专项计划项目 |
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| 摘 要： | 对筛选的5株大肠杆菌(E2:O2、E6:O78、E7:O8、E9:O9、E22:O138)进行药敏试验以及致病性大肠杆菌原生质的制备、融合和筛选试验。结果表明:①5株大肠杆菌对多种抗菌素存在抗药性,并且这种抗药性能进行培育,同时,在原生质的融合过程中能递进;②大肠杆菌原生质体选择的最佳组合是:溶菌酶2.0 g/L,处理时间是20 m in;③大肠杆菌的融合率在10-8左右,大肠杆菌之间的融合率为10-6左右;共选出3株融合菌株为E2-E6、E6-E7和E2-E22,各融合株的生化特征介于起源菌株的生化特征之间,有2株融合株的抗原特征含原菌株各自的抗原成分。
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| 关 键 词： | 致病性大肠杆菌 苗用菌株 原生质体融合 |
Study on Protoplast Fusion of Vaccine Use Strain on the Pathogenic Escherichia Coli |
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| GOU Xiang-zhen,DANG Yan,SUN Chun-xiang,MA Zhi-hong,YANG Zeng-qi,WANG Jing-jue,CHEN De-kun.Study on Protoplast Fusion of Vaccine Use Strain on the Pathogenic Escherichia Coli[J].Journal of Animal Science and Veterinary Medicine,2010,29(5):36-39. |
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| Authors: | GOU Xiang-zhen DANG Yan SUN Chun-xiang MA Zhi-hong YANG Zeng-qi WANG Jing-jue CHEN De-kun |
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| Institution: | 1.Gansu Institute of Animal Science and Veterinary Medicine,Pingliang Gansu 744000,China;2.Northwest A＆F University) |
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| Abstract: | In this test,5 strains escherichia colis（E2：O2、E6：O78、E7：O8、E9：O9、 E22：O138）were elected to make drug sensitive experiment and the test of preparation,fusion,and selection on pathogenic escherichia coli protoplasm.The results showed that：①Five strains escherichia coli existed Drug-resistance for various antibiotics and the Drug-resistance could be cultivated,meanwhile it could be progressive on the process of Protoplast Fusion.②The best combination on escherichia protoplast was that the lysozyme and the time were 2.0 g/L and 20 min,respectively.③Fusion rate of Escherichia coli was about 10-8,and fusion rate among the Escherichia coli was about 10-6.Three fusion strains selected were E2-E6,E6-E7 and E2-E22,respectively.The biochemical characteristic of each strain lay between that of the origin strains,and the antigen characteristics of two strains contained antigen components of original strains. |
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| Keywords: | pathogenic escherichia coli vaccine use strain protoplast fusion |
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