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贵州紫苏叶中氨基酸及游离氨基酸含量分析
引用本文:金石诚,刘忠伟,沈奇,李月,牛熙,朱秋劲.贵州紫苏叶中氨基酸及游离氨基酸含量分析[J].贵州大学学报(农业与生物科学版),2016(4):13-17.
作者姓名:金石诚  刘忠伟  沈奇  李月  牛熙  朱秋劲
作者单位:1. 贵州大学 农业生物工程研究院,贵州 贵阳,550025;2. 贵州省油菜研究所,贵州 贵阳,550008;3. 贵州大学 酿酒与食品工程学院,贵州 贵阳,550025
基金项目:贵州特色植物资源研究与开发(浙江大学馥莉食品研究院基金)
摘    要:采用L-8800氨基酸分析仪对奇苏II、奇苏III、M183和M218四个贵州本地紫苏品种叶片中总氨基酸及游离氨基酸进行测定。结果表明,四个品种的贵州紫苏叶中总氨基酸含量有显著差异,最高为奇苏II,鲜叶含量为8.02 g/100g;游离氨基酸中奇苏III与M183两个品种均检出17种游离氨基酸,奇苏II与M218共检出16种及15种游离氨基酸,四个品种紫苏叶游离氨基酸含量在0.25~0.31 g/100g之间。结果还表明,四种贵州紫苏叶中氨基酸EAA/TAA、EAA/NEAA值接近WHO/FHO参考标准,药效氨基酸比例高达60%以上,具有较高的营养价值和保健作用,是有利于人体氨基酸营养平衡的植物氨基酸资源。

关 键 词:紫苏  总氨基酸  游离氨基酸

Amino Acid and Free Amino Acid Composition Analysis in Guizhou Perilla FRUTESCENS Leaf
JIN Shi-cheng;LUI Zhong-wei;SHEN Qi;LI Yue;NIU Xi;ZHU Qiu-jin.Amino Acid and Free Amino Acid Composition Analysis in Guizhou Perilla FRUTESCENS Leaf[J].Journal of Mountain Agriculture & Biology,2016(4):13-17.
Authors:JIN Shi-cheng;LUI Zhong-wei;SHEN Qi;LI Yue;NIU Xi;ZHU Qiu-jin
Institution:JIN Shi-cheng;LUI Zhong-wei;SHEN Qi;LI Yue;NIU Xi;ZHU Qiu-jing;Insitute of Agro-Bioengineering,Guizhou University;Guizhou Rape Institute;School of liquor and food engineering,Guizhou University;
Abstract:Amino acids of fourlocalvarieties, Qisu II, Qisu III, M183 and M218, of Perilla frutescens widely grown in Guizhou were determined by using the L-8800 amino acid analyzer. The results showed that the total amino acid content in the four varieties of Perilla frutescens leaf were significantly different the highest is QisuII, fresh leaf amino acid con-tent is 8.02 g/100g; There were 17 kinds offree amino acid detectedin QisuIII and M183, while in Qisu IIand M218, there were 16 and 15 kinds of free amino aciddetected respectively.The free amino acid content of the four varieties’ leaf was varied between 0.25 g/100g and 0.31 g/100g. Results also showed that ratios of essential amino acid to total amino acid and non-essential amino acid, EAA/TAA, EAA/NEAA,in the four varieties’ leaf close to the reference standard-sratios put forword by WHO/FHO, efficacy amino acid ratio as high as more than 60%.
Keywords:Perilla frutescens  amino acid  free amino acid
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