| 基于ITS-1序列的部分鳚亚目鱼类的分子系统进化关系研究 |
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| 引用本文： | 张源真,王伟,姜志强.基于ITS-1序列的部分鳚亚目鱼类的分子系统进化关系研究[J].现代农业科技,2012(8):318-320. |
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| 作者姓名： | 张源真 王伟 姜志强 |
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| 作者单位： | 大连海洋大学,农业部北方海水增养殖学重点实验室,辽宁大连116023 |
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| 基金项目： | 辽宁省重大科技攻关项目，辽宁省教育厅计划 |
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| 摘 要： | 通过PCR扩增获得了3种中国黄渤海海域的鳚亚目鱼类的线粒体ITS-1基因,并以条斑马鲛为外群,生成供系统发育分析的序列矩阵,利用MEGA 4.0软件分析序列的碱基组成、差异百分比、转换/颠换值等,应用最大简约法(MP)和邻接法(NJ)构建系统发育树。结果表明:在鳚亚目鱼类的ITS-1片段生成的序列矩阵中发现有碱基的插入缺失现象,共有49 bp变异位点,转换/颠换值为1.0;3种鱼ITS-1长度为375~562 bp,其中方氏云鳚的最长,为532~562 bp,六线鳚的最短,为375 bp;锦鳚科的方氏云鳚和云鳚聚为一支;方氏云鳚和云鳚种间遗传距离只有0.02,亲缘关系最近。
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| 关 键 词： | 鳚亚目 线粒体DNA ITS-1基因序列 系统发育 |
Research on Molecular Phylogenetic Relationship of Partial Blennioidei Based on ITS-1 Sequences |
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| Authors: | ZHANG Yuan-zhen WANG Wei JIANG Zhi-qiang |
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| Institution: | (Key Laboratory of Northern Mariculture,Ministry of Agriculture,Dalian Ocean University,Dalian Liaoning 116023) |
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| Abstract: | In this study,the internal transcribed spacer 1(ITS-1)partial sequences of 3 species from Blennioidei in China yellow and bohai seas were amplified using PCR technique,Scomberomorus regalis was designed as outgroup.Nucleotide composition frequencies,Kimura-2 parameter genetic distance and transition/transversion ratios were analyzed with MEGA 4.0 software.Molecular phylogenetic trees were constructed by maximum parsimony(MP)and neighborjoining(NJ)methods also with MEGA 4.0.The results were as follows:There were insertions and deletions in the aligned base pairs,49 bp variable sites were found in the analysis matrix.,the average ts/tv ratio was 1.0;The length of ITS1 was from 375 bp to 562 bp,with the Enedrias fangi′s the longest,and the Ernogrammus hexagrammus the shortest.Enedrias nebulosus and Enedrias fangi were placed in a same clade with support of a high bootstrap value.Enedrias fangi and Enedrias nebulosus had a close relationship,as the genetic distance between the two species of ITS-1 was only 0.02. |
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| Keywords: | Blennioidei mitochondrial DNA(mtDNA) internal transcribed spacer 1(ITS-1) molecular phylogeny |
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