| 穗花杉组织培养初探 |
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| 引用本文： | 喻晓雁,刘克旺,梁文斌.穗花杉组织培养初探[J].中南林业科技大学学报,2005,25(2):55-58. |
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| 作者姓名： | 喻晓雁 刘克旺 梁文斌 |
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| 作者单位： | 浙江医药高等专科学校 浙江宁波315100
(喻晓雁)，中南林学院 湖南株洲412006
(刘克旺)，中南林学院 湖南株洲412006(梁文斌) |
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| 摘 要： | 穗花杉雌雄异株,繁殖率低,属珍稀濒危植物.为了实现试管保存穗花杉种质资源,以其嫩茎为材料,探讨了穗花杉试管芽生芽方法,即试管微型扦插方法.结果表明:腋芽分化的最适培养基为MB(1/2MS B5) KT1.5 mg/L NAA0.5 mg/L 蔗糖2.0% 琼脂0.8%,腋芽诱导率可达60%;诱导不定根最适培养基为1/4MS IBA4 mg/L NAA2 mg/L 琼脂0.8% 蔗糖2.0%.
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| 关 键 词： | 生物技术 穗花杉 试管微型扦插 芽生芽 组织培养 |
| 文章编号： | 1000-2502(2005)02-0055-04 |
| 修稿时间： | 2004年9月6日 |
A Brief Study of Cuttage in Vitro of Amentotaxus argotaenia |
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| YU Xiao-yan,LIU Ke-wang,LIANG Wen-bing.A Brief Study of Cuttage in Vitro of Amentotaxus argotaenia[J].Journal of Central South Forestry University,2005,25(2):55-58. |
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| Authors: | YU Xiao-yan LIU Ke-wang LIANG Wen-bing |
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| Abstract: | Amentotaxus argotaenia lies only in China, and its population is reduced so sharply that it faces the danger of extinction. Its rapid reproduction is studied for preserving the species in vitro. Through the process of sterilizing the Cuttage of Amentotaxus argotaenia with multiple methods, making it in different light and temperature, inducing axillary buds in different medium, strengthening the buds and inducing its roots in vitro, we get some full seedings successfully. The results show that axillary buds can be induced successfully from yearly shoot cutting in culture and the optimum medium is: MB (1/2MS+ B5)+ KT1.5 mg/L + NAA0.5 mg/L + sucrose 2.0% + agar 0.8% . The rate of success is about 60%. The seedlings in vitro take root successfully in the medium 1/4MS + IBA4 mg/L+ NAA2 mg/L + agar 0.8% + sucrose 2.0% . |
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