Protein Phosphorylation and Protein Patterns in Developing Endosperms of Barley |
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| Authors: | T Yupsanis P R Shewry |
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| Institution: | Aristotelian University of Thessaloniki, School of Chemistry, Laboratory of Biochemistry, GR-54006 Thessaloniki, Greece;Department of Agricultural Sciences, University of Bristol, Long Ashton Research Station, Bristol BS18 9AF, England. |
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| Abstract: | The SDS-soluble protein-fractions of barley endosperms (2 and 3 weeks after anthesis) were compared using 1-D SDS-PAGE and 2-D IEF/SDS-PAGE. Similar procedures have been followed after in vivo phosphorylation of isolated endosperm with 32PO4.Protein patterns were similar when 1-D SDS-PAGE was used. On the other hand, 2-D IEF/SDS-PAGE revealed several major differences, the most important being the appearance of a major protein of Mr about 40,000 in the 3-weeks old endosperm. By contrast, the patterns of phosphorylated proteins, with both methods, were relatively simple. Only two phosphorylated bands showed up in the 3-weeks old endosperm: a highly labelled band of Mr about 65,000, and a band of about 75,000. In the case of 2-weeks old endosperm, where the phosphorylation was more active, these and several others were also labelled, but the Mr 75,000 band was labelled more intensely. |
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| Keywords: | Hordeum vulgare grain development endosperm 1-D SDS-PAGE 2-D IEF/SDS-PAGE storage proteins phosphorylation |
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