| 具几丁质酶抑制活性的中性蛋白酶的筛选与纯化 |
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| 引用本文： | 张新军,谢洁,蓝希钳,肖杰,潘国庆,周泽扬.具几丁质酶抑制活性的中性蛋白酶的筛选与纯化[J].西南农业大学学报,2006,28(3):513-517. |
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| 作者姓名： | 张新军 谢洁 蓝希钳 肖杰 潘国庆 周泽扬 |
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| 作者单位： | 西南大学农业部蚕学重点实验室微生物研究中心，西南大学农业部蚕学重点实验室微生物研究中心，西南大学农业部蚕学重点实验室微生物研究中心，西南大学农业部蚕学重点实验室微生物研究中心，西南大学农业部蚕学重点实验室微生物研究中心，西南大学农业部蚕学重点实验室微生物研究中心 重庆400716，重庆400716，重庆400716，重庆400716，重庆400716，重庆400716，重庆师范大学动物生物学研究室，重庆400047 |
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| 摘 要： | 利用平板快速筛选法,从土壤中筛选到一株细菌BacillusSp.2108,其代谢物对几丁质酶具有较强的抑制活性。对其发酵液离心,用硫酸铵盐析后,经Sephadex G-25脱盐柱脱盐、DEAE Sephrose Fast F low阴离子交换柱层析和低压液相色谱Superdex G 75凝胶过滤层析等步骤进行分离纯化,最后通过SDS-PAGE检测,得到一条单带。纯品经SDS-PAGE,转膜,测序,及序列比对,初步判定此抑制物是一种中性蛋白酶。
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| 关 键 词： | 几丁质酶 几丁质酶抑制物 纯化 层析 中性蛋白酶 |
| 文章编号： | 1000-2642（2006）03-0513-05 |
| 收稿时间： | 02 27 2005 12:00AM |
| 修稿时间： | 2005-02-27 |
SCREENING AND PURIFICATION OF NEUTRAL PROTEASE WITH CHITINASE INHIBITOR ACTIVITY |
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| ZHANG Xin-jun,XIE Jie,LAN Xi-qian,XIAO Jie,PAN Guo-qing,ZHOU Ze-yang.SCREENING AND PURIFICATION OF NEUTRAL PROTEASE WITH CHITINASE INHIBITOR ACTIVITY[J].Journal of Southwest Agricultural University,2006,28(3):513-517. |
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| Authors: | ZHANG Xin-jun XIE Jie LAN Xi-qian XIAO Jie PAN Guo-qing ZHOU Ze-yang |
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| Institution: | 1. The Key Sericulture Laboratory of Agricultural Ministry, Southwest University, Chongqing 400716, China; 2. College of Bioscience, Chongqing Normal University, Chongqing 400047, China |
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| Abstract: | By a rapid plate screening procedure,a strain with chitinase inhibitor activity was obtained from soil samples taken from different places.The targeted metabolite with chitinase inhibitor activity was further purified from the culture fluid by fractional precipitation with ammonium sulfate,chromatography on Sephdex G-25 gel filtration and DEAE Sepharose Fast Flow anion exchange chromatography,and finally gel filtration on Superdex G 75.SDS-PAGE of the purified protein displayed its characteristics and high purity.Ten N-terminal amino acids of the protein were sequenced by PVDF transblotting,and search of Blast in GenBank suggested that this protein is a kind of neutral protease. |
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| Keywords: | chitinase chitinase inhibitor purification chromatography neutral proteinase |
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