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| 用二次回归正交旋转组合设计优化桑叶多酚的提取工艺 | |
| 引用本文： | 沈维治,廖森泰,刘吉平,邹宇晓,李妍.用二次回归正交旋转组合设计优化桑叶多酚的提取工艺[J].蚕业科学,2009,35(3). |
| 作者姓名： | 沈维治 廖森泰 刘吉平 邹宇晓 李妍 |
| 作者单位： | 1. 华南农业大学动物科学学院,广州,510642;广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室,广州,510610 2. 华南农业大学动物科学学院,广州,510642 3. 广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室,广州,510610 |
| 基金项目： | 广东省科技厅社会发展攻关项目，广州市科技攻关项目 |
| 摘 要： | 桑叶多酚有消除机体内自由基毒性的作用,为桑叶中具有药用和保健功能开发价值的成分之一。运用二次回归正交旋转组合设计法,研究了桑叶多酚提取工艺中原料溶剂乙醇的浓度,以及原料的质量浓度、原料溶液pH值、提取次数等因素对桑叶多酚得率的影响,建立了具有良好预测性能的提取条件数学模型,确定了最优提取工艺条件。试验结果表明,在桑叶多酚提取工艺条件各因素中,对桑叶多酚得率影响作用大小的顺序为原料溶液pH值>原料的质量浓度>溶剂乙醇的浓度。最优提取工艺条件为:以70%乙醇作原料溶剂,原料质量浓度0.025g/mL,原料溶液pH值4。在此优化工艺条件下,从桑叶中提取桑叶多酚的质量比为16.11 mg/g。 |
| 关 键 词： | 桑叶 多酚 二次回归正交旋转组合设计 提取工艺 |
Optimization of Polyphenol Extraction From Mulberry Leaves Using Quadratic Regression Rotational Combinational Design |
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| SHEN Wei-Zhi,LIAO Sen-Tai,LIU Ji-Ping,ZOU Yu-Xiao,LI Yan.Optimization of Polyphenol Extraction From Mulberry Leaves Using Quadratic Regression Rotational Combinational Design[J].Acta Sericologica Sinica,2009,35(3). | |
| Authors: | SHEN Wei-Zhi LIAO Sen-Tai LIU Ji-Ping ZOU Yu-Xiao LI Yan |
| Institution: | SHEN Wei-Zhi1,2 LIAO Sen-Tai1,2* LIU Ji-Ping1 ZOU Yu-Xiao2 LI Yan1,2 (1College of Animal Science,South China Agricultural University,Guangzhou 510642,China 2The Sericulture & Farm Produce Processing Research Institute,Guangdong Academy of Agriculture Sciences,Key Laboratory of Agro-food Science , Technology of Guangdong,Guangzhou 510610,China) |
| Abstract: | Being capable of eliminating toxicity of free radicals in organisms,the polyphenols from mulberry leaves are one of the valuable components which can be developed for use of medicinal and health-care functions.By means of quadratic regression rotational combinational design,the effect of ethanol concentration,material mass concentration,pH of material solution and extraction times on the yield of polyphenol from mulberry leaves was studied.A mathematical model of extraction conditions with good prediction p... |
| Keywords: | Mulberry leaf Polyphenol Quadratic regression rotational combination design Extraction process |
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