Marker-assisted increase of genetic diversity in a double-low seed quality winter oilseed rape genetic background |
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| Authors: | P Basunanda T H Spiller M Hasan A Gehringer J Schondelmaier W Lühs W Friedt and R J Snowdon |
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| Institution: | Department of Plant Breeding, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany;;Saaten-Union Resistenzlabor GmbH, Hovedisser Straße 92, D-33818 Leopoldshoehe, Germany;;Corresponding author, E-mail: |
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| Abstract: | Generation of novel genetic diversity for maximization of heterosis in hybrid production is a significant goal in winter oilseed rape breeding. Here, we demonstrate that doubled haploid (DH) production using microspore cultivation can simultaneously introgress favourable alleles for double‐low seed quality (low erucic acid and low‐glucosinolate content) into a genetically diverse Brassica napus genetic background. The DH lines were derived from a cross between a double‐low quality winter rapeseed variety and a genetically diverse semisynthetic B. napus line with high erucic acid and high glucosinolates (++ quality). Twenty‐three low‐glucosinolate lines were identified with a genome component of 50–67% derived from the ++ parent. Four of these lines, with a genome component of 50–55% derived from the ++ parent, also contained low erucic acid. Heterosis for seed yield was confirmed in test‐crosses using these genetically diverse lines as pollinator. The results demonstrate the potential of marker‐assisted identification of novel genetic pools for breeding of double‐low quality winter oilseed rape hybrids. |
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| Keywords: | Brassica napus Oilseed rape double-low quality quantitative trait locus QTL heterosis |
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