| 鸡致病性大肠杆菌I型菌毛交叉保护性研究及其结构基因 FimA 的序列分析 |
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| 引用本文： | 隋兆峰,刘文强,范伟兴,许传田,赵宏坤.鸡致病性大肠杆菌I型菌毛交叉保护性研究及其结构基因 FimA 的序列分析[J].中国预防兽医学报,2003,25(6):440-444. |
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| 作者姓名： | 隋兆峰 刘文强 范伟兴 许传田 赵宏坤 |
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| 作者单位： | 山东农业大学,动物科技学院,山东,泰安,271018 |
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| 基金项目： | 山东省自然科学基金资助项目 (Y98D170 6 5 ) |
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| 摘 要： | 从山东省各地分离到107株鸡致病性大肠杆菌，选择了其中2株高致病性大肠杆菌，血清型分别为O78和OM，经增菌培养后提取菌毛制备为单价菌毛油乳苗，分别接种于1日龄和14日龄雏鸡，于4周龄攻毒。结果二者之间有一定的交叉保护作用。根据Genbank收录的人源大肠杆菌I型菌毛FimA基因和P型菌毛papA基因序列，分别设计了2对引物。通过PCR对上述2株大肠杆菌进行扩增，结果只有FimA的一对引物扩增出相应的条带，经测序证明为FimA基因。papA基因的引物未扩出任何条带，证明这2株大肠杆菌表达I型菌毛。通过对2株大肠杆菌结构基因FimA进行分析，发现二者具有高度的同源性。本研究的目的是探讨菌毛亚单位之间的交叉保护性与其菌毛的结构基因之间是否存在相关性。
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| 关 键 词： | 大肠杆菌 菌毛 免疫 交叉保护 FimA |
| 文章编号： | 1008-0589(2003)06-0440-04 |
| 修稿时间： | 2003年1月28日 |
Cross protection of type I fimhriae vaccine of Avian pathogenic E.coli and nucleoutide sequence analysis of FimA gene |
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| SUI Zhao-feng,LIU Wen-qiang,FAN Wei-xing,XU Chuan-tian,ZHAO Hong-kun.Cross protection of type I fimhriae vaccine of Avian pathogenic E.coli and nucleoutide sequence analysis of FimA gene[J].Chinese Journal of Preventive Veterinary Medicine,2003,25(6):440-444. |
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| Authors: | SUI Zhao-feng LIU Wen-qiang FAN Wei-xing XU Chuan-tian ZHAO Hong-kun |
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| Abstract: | avian pathogenic E.coli isolates were isolated from shandong province.Two high pathogenic E.coli isolates were used for preparing the fimbriae vaccines.The chicken were vaccined on 1 day-old and 14 day-old,respectively.Then challenged via the air sac with E.coli(O_(78),O_(24))on 4 week-old chickens.The result was that the two isolates had certain cross protection.According to the published type 1 and type P nucleotide sequence from human E.coli,two pairs of primers were designed and synthesized.Two positive 549 bp products were amplificated by polymerse chain reaction(PCR) using the primers of FimA from the two strains.No amplification were obtained by using the primers of papA.There is high homology between FimA gene of the two isolates.The aim of the study is to make clear if there is relativity between cross protection and structural gene of fimbriae. |
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| Keywords: | E coli fimbriae immune cross protection FimA |
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