| 两种常用激素组合下棉花体细胞胚胎发生过程的组织学观察 |
| |
| 引用本文： | 朱华国,张献龙,金双侠,刘冠泽.两种常用激素组合下棉花体细胞胚胎发生过程的组织学观察[J].棉花学报,2012,24(2):159-166. |
| |
| 作者姓名： | 朱华国 张献龙 金双侠 刘冠泽 |
| |
| 作者单位： | 1. 石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,石河子,832003
2. 华中农业大学植物科学技术学院/作物遗传改良国家重点实验室,武汉,430070 |
| |
| 基金项目： | 国家棉花产业技术体系项目;石河子大学动植物育种专项;石河子大学高层次人才项目(RCZX200905);兵团博士资金项目(2011BB004) |
| |
| 摘 要： | MSB培养基添加两种常用植物激素组合，Indole-3-butyric acid (IBA) + kinetin (KT)和2, 4-dichlorophenoxyacetic acid (2, 4-D) + KT，分别命名为IK和DK处理用来诱导陆地棉体细胞胚胎发生。陆地棉品系YZ1下胚轴切段作为外植体在诱导培养基上培养4周后，继代到分化培养基上促进体细胞胚胎发生。结果表明，两种处理均有体细胞胚胎发生，其中IK处理上外植体嫁接33 d后就可见体细胞胚出现，分化率高达97.6%，大多数外植体表现为体细胞胚较胚性愈伤早出现。DK处理中体细胞胚胎发生慢，体细胞胚都是经过明显的胚性愈伤发育而来，胚性愈伤分化率明显低于IK处理，仅为28.6%。此外，IK处理中的外植体在培养过程中大部分都出现了须根，而DK处理中则没有。两种处理中出现了两种不同的体细胞胚胎发生过程，组织学观察结果表明DK和IK处理中的体细胞胚分别主要起源于初生形成层和皮层。
|
| 关 键 词： | 棉花 体细胞胚胎发生 组织学观察 植物生长调节剂 |
| 收稿时间： | 2011-03-30; |
Histological Observation of the Processes during Somatic Embryogenesis in the Two Most Commonly Used PGR Regimes in Cotton |
| |
| ZHU Hua-guo
,
ZHANG Xian-long
,
JIN Shuang-xia
,
LIU Guan-ze.Histological Observation of the Processes during Somatic Embryogenesis in the Two Most Commonly Used PGR Regimes in Cotton[J].Cotton Science,2012,24(2):159-166. |
| |
| Authors: | ZHU Hua-guo ZHANG Xian-long JIN Shuang-xia LIU Guan-ze |
| |
| Institution: | 1.Shihezi University Agricultural College/ The Key Laboratory of Oasis Eco-agriculture,Xinjiang Production and Construction,Shihezi 832003,China;2.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China) |
| |
| Abstract: | The two most commonly used plant growth regulator regimes(PGR) are the IK and DK treatments for somatic embryogenesis.Segments from hypocotyls of Gossypium hirsutum cv.YZ-1 were cultured on callus induction medium for 4 weeks,and sub-cultured on differentiation medium for somatic embryogenesis.The results demonstrated that somatic embryogenesis occurred in the two treatments.In the IK treatment,somatic embryos appeared on day 33 after inoculation,the ratio of differentiation was 97.6%,and somatic embryos commonly appeared prior to an obvious embryogenic callus.One the other hand,somatic embryogenesis was represented at a late stage in the DK treatment,somatic embryos developed from obvious embryogenic calli,and the ratio of callus differentiation was distinctly lower than that with IK treatment,being only 28.6%.Furthermore,adventitious roots almost appeared on the surface of all explants in the IK treatment,but none were found in the DK treatment.Therefore,two distinct patterns of somatic embryogenesis occurred in the two treatments,with histological observation suggesting that the majority of somatic embryos originated from primary meristem and cortex in the DK and the IK treatments,respectively. |
| |
| Keywords: | cotton somatic embryogenesis histological observation plant growth regulator |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《棉花学报》浏览原始摘要信息 |
| 点击此处可从《棉花学报》下载免费的PDF全文 |
|
