| 小鹅瘟病毒H株VP3基因序列分析 |
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| 引用本文： | 王彬,孙德君,柴华,王丹娜,潘兴广.小鹅瘟病毒H株VP3基因序列分析[J].中国畜牧兽医,2010,37(10):155-158. |
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| 作者姓名： | 王彬 孙德君 柴华 王丹娜 潘兴广 |
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| 作者单位： | （哈药集团生物疫苗有限公司研发中心，哈尔滨 150069） |
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| 摘 要： | 试验利用PCR技术从自行分离的GPV基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段,并与pMD18-T载体连接,转化大肠杆菌DH 5α感受态细胞,提取重组质粒经PCR和酶切鉴定后,对插入片段进行序列测定及分析。结果表明,VP3基因全长1605 bp,编码534个氨基酸;分子生物学分析表明所分离到的病毒为强毒株。
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| 关 键 词： | 小鹅瘟病毒 VP3基因 序列分析 |
Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain |
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| WANG Bin,SUN De-jun,CHAI Hua,WANG Dan-na,PAN Xing-guang.Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain[J].China Animal Husbandry & Veterinary Medicine,2010,37(10):155-158. |
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| Authors: | WANG Bin SUN De-jun CHAI Hua WANG Dan-na PAN Xing-guang |
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| Institution: | (Harbin Phermaceutical Group Bio-vaccine Co.Ltd., R and D Center, Harbin 150069, China) |
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| Abstract: | VP3 gene of goose parvovirus was amplified from the DNA by polymerase chain reaction(PCR), the VP3 gene was cloned into pMD18-T vector. The recombinant plasmid was indentified with restriction endonuclease analysis and PCR, and then sequenced. The result of sequence analysis showed that VP3 gene had 1605 bp and included a complete open reading frame which encoding a protein of 534 amino acids, and the analysis of molecular biology showed that the virus was virulent strain. |
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| Keywords: | GPV VP3 gene sequence analysis |
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