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| 高效液相色谱荧光检测法同时检测鸡蛋中氟苯尼考及其代谢物氟苯尼考胺残留 | |
| 引用本文： | 谢恺舟,姚宜林,徐东,陈学森,谢星,戴国俊,刘宗平,陈国宏.高效液相色谱荧光检测法同时检测鸡蛋中氟苯尼考及其代谢物氟苯尼考胺残留[J].中国兽医杂志,2011,47(8). |
| 作者姓名： | 谢恺舟 姚宜林 徐东 陈学森 谢星 戴国俊 刘宗平 陈国宏 |
| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏扬州225009;江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 2. 南京农业大学动物医学院,江苏南京,210095 3. 扬州大学兽医学院,江苏扬州,225009 |
| 基金项目： | 江苏省高新技术计划项目(BG2005307); 江苏省科技成果转化专项(BA2008071) |
| 摘 要： | 建立了高效液相色谱荧光检测法同时检测鸡蛋中氟苯尼考及其代谢物氟苯尼考胺残留的方法.鸡蛋经碱性乙酸乙酯提取,饱和正己烷脱脂,氮吹仪吹干浓缩后用流动相溶解.用Lichrospher C1s柱,以乙腈-磷酸二氢钠溶液(0.01 mol/L,含0.005 mol/L十二烷基硫酸钠和0.1％三乙胺)(35:65,v/v)为流动相,在激发波长224 nm、发射波长285 nm处用荧光检测器检测.氟苯尼考在0.01～10.0 mg/L、氟苯尼考胺在0.002 5～2.5 mg/L浓度范围内,本方法线性关系良好,相关系数分别为0.99 97和0.999 8.当添加水平氟苯尼考为15～500μg/kg、氟苯尼考胺为5～500 μg/kg时,该方法平均回收率分别为87.41％～92.29％和89.01％～95.15％,相对标准偏差分别为3.51％～5.36％和4.28％～5.72％;检测限分别为1.5 μg/kg和0.5 μg/kg(S/N=3);定量限分别为5μg/kg和2 μg/kg(S/N=10).该方法简便、快速、灵敏度高,可同时检测鸡蛋中氟苯尼考和氟苯尼考胺的残留. |
| 关 键 词： | 高效液相色谱 荧光检测法 氟苯尼考 氟苯尼考胺 鸡蛋 残留 |
Simultaneous determination of residues of forfenicol and the metabolite forfenicol amine in hen eggs by HPLC with fluorescence detection |
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| XIE Kai-zhou,YAO Yi-lin,XU Dong,CHEN Xue-sen,XIE Xing,DAI Guo-jun,LIU Zong-ping,CHEN Guo-hong.Simultaneous determination of residues of forfenicol and the metabolite forfenicol amine in hen eggs by HPLC with fluorescence detection[J].Chinese Journal of Veterinary Medicine,2011,47(8). | |
| Authors: | XIE Kai-zhou YAO Yi-lin XU Dong CHEN Xue-sen XIE Xing DAI Guo-jun LIU Zong-ping CHEN Guo-hong |
| Institution: | XIE Kai-zhou1,2,YAO Yi-lin1,XU Dong1,CHEN Xue-sen1,XIE Xing3,DAI Guo-jun1,LIU Zong-ping4,CHEN Guo-hong1,2(1.College of Animal Science and Technology,YangZhou University,Yangzhou 225009,China,2.Key Laboratory for Animal Genetic,Breeding,Reproduction and Molecular Design of Jiangsu Province,3.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,4.College of Veterinary Medicine,Yangzhou University,China) |
| Abstract: | A high performance liquid chromatography with fluorescence detection(HPLC-FLD)method was developed for simultaneous determination of residues of forfenicol(FF)and the metabolite forfenicol amine(FFA) in eggs of laying hens.FF and FFA were extracted from eggs of laying hens with alkalized ethyl acetate.The extract solution was rinsed with n-hexane saturated with acetonitrile and dried with nitrogen evaporator and residues were dissolved in mobile phase.FF and FFA were separated on a Lichrospher C18 column us... |
| Keywords: | HPLC fluorescence detection florfenicol(FF) florfenicol amine(FFA) hen eggs residue |
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