| 巨型艾美耳球虫gam82基因核酸疫苗的构建与免疫原性分析 |
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| 引用本文： | 彭昊,许金俊,刘丹丹,李建梅,陶建平.巨型艾美耳球虫gam82基因核酸疫苗的构建与免疫原性分析[J].中国兽医杂志,2011,47(9). |
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| 作者姓名： | 彭昊 许金俊 刘丹丹 李建梅 陶建平 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州,225009 |
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| 基金项目： | 江苏省自然科学基金(B2007079) |
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| 摘 要： | 以重组质粒pGEM-T-gam82为模板,构建巨型艾美耳球虫gam82基因核酸疫苗.采用MTT法与ELISA对该核酸疫苗接种鸡体后淋巴细胞增殖与抗体水平的变化进行检测,结果显示,该核酸疫苗具有一定的免疫原性.
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| 关 键 词： | 巨型艾美耳球虫 gam82基因 核酸疫苗 免疫原性 |
Construction of Eimeria maxima gam82 gene nucleic-acid vaccine and analysis of its immunogenicity |
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| PENG Hao,XU Jin-jun,LIU Dan-dan,LI Jian-mei,TAO Jian-ping.Construction of Eimeria maxima gam82 gene nucleic-acid vaccine and analysis of its immunogenicity[J].Chinese Journal of Veterinary Medicine,2011,47(9). |
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| Authors: | PENG Hao XU Jin-jun LIU Dan-dan LI Jian-mei TAO Jian-ping |
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| Institution: | PENG Hao,XU Jin-jun,LIU Dan-dan,LI Jian-mei,TAO Jian-ping (College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | Using recombination plasmid pGEM-T-gam82 as template,the vaccine pcDNA-gam82 of E.maxima was constructed.To evaluate the efficacy of the vaccine,lymphocyte proliferation assay and antibody determination was carried out.The results showed that chickens immunized with the vaccine produced significantly higher lymphocyte proliferation and antibody responses than the control birds. |
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| Keywords: | Eimeria maxima gam82 gene nucleic-acid vaccine immunogenicity |
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