| 转甘蔗抗虫基因CpTI的RT-PCR检测 |
| |
| 引用本文： | 刘洪博,姚丽,苏火生,刘新龙,应雄美,蔡青,吴才文.转甘蔗抗虫基因CpTI的RT-PCR检测[J].中国糖料,2011(1):11-12. |
| |
| 作者姓名： | 刘洪博 姚丽 苏火生 刘新龙 应雄美 蔡青 吴才文 |
| |
| 作者单位： | 1. 云南省农业科院甘蔗研究所,开远,661600;云南省甘蔗遗传改良重点实验室,开远,661600
2. 云南省甘蔗遗传改良重点实验室,开远,661600;云南省农业科学院生物技术与种质资源研究所,昆明,650223 |
| |
| 基金项目： | 国家科技支撑计划育种项目，国家863计划 |
| |
| 摘 要： | 利用已修饰的CpTI基因进行转基因检测,根据修饰后的目的基因片段序列对两个甘蔗品种(P44和福农94-0403)的转基因甘蔗进行RT-PCK检测研究,结果在被检测的10 株甘蔗中验证了3株已转入植株中,RT-PCR检测到的20、25、26号植株均呈阳性,证明被修饰的sck基因已成功转入甘蔗.
|
| 关 键 词： | 甘蔗 抗虫基因 RT-PCR检测 |
RT-PCR Detection on Transgenic Sugarcane for Insect Resistant Gene CpTI |
| |
| LIU Hong-bo,YAO Li,SUN Huo-sheng,LIU Xin-long,YING Xiong-mei,CAI Qing,WU CAI-wen.RT-PCR Detection on Transgenic Sugarcane for Insect Resistant Gene CpTI[J].Sugar Crops of China,2011(1):11-12. |
| |
| Authors: | LIU Hong-bo YAO Li SUN Huo-sheng LIU Xin-long YING Xiong-mei CAI Qing WU CAI-wen |
| |
| Institution: | LIU Hong-bo1,2,YAO Li1,SUN Huo-sheng1,LIU Xin-long1,YING Xiong-mei1,CAI Qing2,3,WU CAI-wen1,2(1.Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan 661600,China,2.Yunnan Key Laboratory of Sugarcane Genetic Improvement,(3.Biotechnology & Genetic Resource Institute,Kunming 650223,China) |
| |
| Abstract: | RT-PCR technique was used to detect whether the reconstructed CpTI gene transferred into two sugarcane cultivars(P44 and Funong94-0403) in the study.The result showed three accessions named as No.20,No.25 and No.26 represented positive of ten accessions,and the size of band detected in positive accessions was about 415 bp,which indicated the reconstructed CpTI gene has been transferred into the two sugarcane cultivars. |
| |
| Keywords: | sugarcane insect resistance gene RT-PCR detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
