| 花叶络石组织培养研究 |
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| 引用本文： | 郭翔,秦国权,李春辉,刘黾.花叶络石组织培养研究[J].广东林业科技,2016,32(6):54-58. |
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| 作者姓名： | 郭翔 秦国权 李春辉 刘黾 |
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| 作者单位： | 岭南园林股份有限公司,广东 东莞,532125 |
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| 基金项目： | 广东省农业领域科技计划资助项目“园林彩叶植物引种驯化与快繁技术研究”(2013B020410005)。 |
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| 摘 要： | 文章以花叶络石（Trachelospermum jasminoides ‘Variegatum’）幼嫩茎段为外植体，研究了不同消毒时间的外植体消毒效果、激素质量浓度配比对腋芽增殖及生根的影响和移栽基质配比，建立花叶络石快速繁殖体系。结果表明：0．1％升汞消毒10 min 效果最好，成活率达72％；花叶络石腋芽增殖较好的培养基组合为 MS ＋BA2．0 mg／L ＋NAA0．05 mg／L，增殖倍数4．23；最佳的生根培养基为1／2MS ＋IBA0．3 mg／L，生根率达到97．2％；花叶络石移栽较优的基质配比为蛭石∶珍珠岩∶泥炭＝6∶1∶3，成活率达93％。
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| 关 键 词： | 花叶络石 茎段 组织培养 快速繁殖 |
| 收稿时间： | 2016/11/18 0:00:00 |
| 修稿时间： | 2016/11/18 0:00:00 |
In Vitro Propagation of Trachelospermum jasminoides ‘Variegatum ’ |
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| GUO Xiang,QIN Guoquan,LI Chunhui and LIU Mian.In Vitro Propagation of Trachelospermum jasminoides ‘Variegatum ’[J].Forestry Science and Technology of Guangdong Province,2016,32(6):54-58. |
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| Authors: | GUO Xiang QIN Guoquan LI Chunhui and LIU Mian |
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| Institution: | Lingnan Landscape Co.,Ltd.,Lingnan Landscape Co.,Ltd.,Lingnan Landscape Co.,Ltd.,Lingnan Landscape Co.,Ltd. |
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| Abstract: | The axillary buds of Trachelospermum jasminoides ‘Variegatum’were selected as the research object in this study.The effects of explants disinfected at different disinfection time,the effects of hormone concentration on axillary bud proliferation and rooting and the transplanting medium were studied in order to estabilish a rapid propagation system.The results showed that the best sterilization time of 0.1% HgCl2 was 10 minutes,the rate of survival reached 72%.The optimum multiplication medium was MS +BA2.0 mg/L +NAA0.05 mg/L,the multiplication rate was 4.23.The best rooting medium was 1 /2 MS +NAA0.3 mg/L,and the rooting rate reached 97.2%.The optimal transplantation medium was vermiculite∶perlite∶peat =6∶1∶3 and the survival rate was 93%. |
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| Keywords: | Trachelospermum jasminoides ‘Variegatum’ nodal explants tissue culture rapid propagation |
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