| 五味子提取物的抑菌和杀菌活性研究 |
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| 引用本文： | 王锦文,边才苗.五味子提取物的抑菌和杀菌活性研究[J].安徽农业科学,2008,36(16):6799-6800. |
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| 作者姓名： | 王锦文 边才苗 |
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| 作者单位： | 台州学院生命科学学院,浙江临海,317000 |
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| 摘 要： | 目的]探讨五味子提取物的抑菌活性,为其合理开发提供依据。方法]采用70%乙醇、60%丙酮及沸水3种溶剂对五味子进行搅拌提取,以金黄色葡萄球菌为供试菌,通过平板二倍稀释法测最低抑菌浓度(MIC),试管二倍稀释法计测最低杀菌浓度(MBC)。结果]五味子3种提取物对金黄色葡萄球菌均有明显的抑制作用,其中60%丙酮提取物的抑制效果最明显,其次是70%乙醇提取物,最后是沸水提取物;MIC分别为25、50和100 mg/ml。同时,低浓度的丙酮提取物抑菌作用不明显,浓度为62.5 mg/ml时细菌的生长被完全抑制,但没有明显的杀菌作用,丙酮提取物的MBC为125 mg/ml。结论]五味子的丙酮提取物具有明显的抑菌和杀菌作用。
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| 关 键 词： | 五味子 提取液 金黄色葡萄球菌 抑菌 |
| 文章编号： | 0517-6611(2008)16-06799-02 |
| 修稿时间： | 2008年3月31日 |
Study on the Bacteriostatic and Bactericidal Activity of Extract from Schisandra Chinensis |
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| WANG Jin-wen.Study on the Bacteriostatic and Bactericidal Activity of Extract from Schisandra Chinensis[J].Journal of Anhui Agricultural Sciences,2008,36(16):6799-6800. |
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| Authors: | WANG Jin-wen |
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| Abstract: | Objectives]The aim was to study the bacteriostatic activities of extract from Schisandra chinensis and provide references for its reasonable development.Method] S.chinensis were stirred and extracted with 3 kinds of solvents such as 70% ethanol,60% acetone and boiling water.With Staphylococcus aureus as tested bacteria,minimumm inhibition concen.(MIC) was determined by agar doubling dilution method and maximum bacteriostatic concen.(MBC) was measured by tube doubling dilution method.Result]Three extracts of S. chinensis had significant anti-microbial activity on S.aureus,that of 60% acetone was most obvious,the second was extracts of 70% ethanol and the last was that of boiling water.Their MIC was 25,50 and 100 mg/ml resp.The bacteriostatic effect of extract of acetone with low concen.was not obvious.The extract at 62.5 mg/ml could inhibit bacterial the growth completely,but had not obvious bactericidal effect.The MBC of extract of acetone was 125 mg/ml.Conclusion]The acetone extract of S.chinensis had obvious bacteriostatic and bactericidal activity. |
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| Keywords: | S chinensis Extract Staphylococcus aureus Bacteriostasis |
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