| 精氨酸对热应激处理的奶牛原代小肠上皮细胞增殖和凋亡的影响 |
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| 引用本文： | 豆梦莹,张才,李元晓,邵琦,朱佳丽,李旺,曹志军.精氨酸对热应激处理的奶牛原代小肠上皮细胞增殖和凋亡的影响[J].畜牧兽医学报,2022,53(2):493-504. |
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| 作者姓名： | 豆梦莹 张才 李元晓 邵琦 朱佳丽 李旺 曹志军 |
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| 作者单位： | 1. 河南科技大学动物科技学院, 洛阳 471023;2. 中国农业大学动物科技学院, 北京 100193 |
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| 基金项目： | 国家自然科学基金(31872537)。 |
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| 摘 要： | 本试验旨在研究精氨酸(L-Arginine,L-Arg)对热应激奶牛原代小肠上皮细胞增殖和凋亡的影响,探究精氨酸对热应激奶牛小肠上皮细胞损伤的修复作用.首先以体外培养的奶牛原代小肠上皮细胞为模型,分为对照组(Con组,37℃)、试验组42℃培养6h后,更换成无精氨酸培养基(HS组)或者不同浓度(2、4、6、8、10 ...
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| 关 键 词： | 精氨酸 奶牛 热应激 小肠上皮细胞 凋亡 |
| 收稿时间： | 2021-04-23 |
Effects of L-Arginine on Proliferation and Apoptosis of Primary Intestinal Epithelial Cells of Dairy Cows Treated with Heat Stress in vitro |
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| DOU Mengying,ZHANG Cai,LI Yuanxiao,SHAO Qi,ZHU Jiali,LI Wang,CAO Zhijun.Effects of L-Arginine on Proliferation and Apoptosis of Primary Intestinal Epithelial Cells of Dairy Cows Treated with Heat Stress in vitro[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):493-504. |
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| Authors: | DOU Mengying ZHANG Cai LI Yuanxiao SHAO Qi ZHU Jiali LI Wang CAO Zhijun |
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| Institution: | 1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China;2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China |
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| Abstract: | The aim of this study was to investigate the effect of L-Arginine(L-Arg)on the proliferation and apoptosis of bovine intestinal epithelial cells injured by heat stress.The bovine primary intestinal epithelial cells cultured in vitro were divided into control group(Con group,37℃)and experimental groups(after being cultured at 42℃for 6 h,the medium without L-Arg(HS group)or the medium with different concentrations(2,4,6,8,10 mmol·L-1)of L-Arg were changed to culture at 37℃for 12 h).The cell viability was detected by CCK-8 and the best healing concentration of L-Arginine was screened.Then the third generation bovine intestinal epithelial cells with good condition were divided into control group(Con group,cultured at 37℃),heat stress group(HS group,cultured at 42℃for 6 h and then cultured at 37℃for 12 h)and 6 mmol·L-1L-Arg group(42℃for 6 h and then replaced with 6 mmol·L-1L-Arg medium at 37℃for 12 h).The morphological changes of cells were observed by DAPI staining,the leakage of lactate dehydrogenase(LDH)was detected by micro method,and the cell cycle was detected by flow cytometry.The relative expression levels of cysteine proteolytic enzyme-3(Caspase-3),cysteine aspartate proteolytic enzyme-9(Caspase-9),Bcl-2-associated X protein(Bax)and B lymphocyte tumor-2(Bcl-2)mRNA and protein were detected by real-time fluorescence quantitative PCR and enzyme-linked immunosorbent assay(ELISA)to evaluate the effect of L-Arg on the proliferation and apoptosis of bovine intestinal epithelial cells injured by heat stress.The results showed that in the range of 2~6 mmol·L-1,compared with HS group,the activity of intestinal epithelial cells was increased by adding L-Arg under heat stress and the activity was the highest when the concentration of L-Arg was 6 mmol·L-1(P<0.05),which could be used as the experimental concentration of L-Arg group in the follow-up experiment.Compared with Con group,the nuclear morphology of HS group was irregular,chromatin was condensed,the leakage of LDH was significantly increased(P<0.05),the ratio of cells at S stage was significantly decreased(P<0.05),the relative expression levels of Bax,Caspase-9,Caspase-3 mRNA and protein were significantly increased(P<0.05),while the protein content of Bcl-2 was significantly decreased in HS group(P<0.05).These results indicated that heat stress promoted the apoptosis of intestinal epithelial cells in dairy cows.Compared with HS group,the nuclear morphology was regular and the leakage of LDH in intestinal epithelial cells of dairy cows treated with 6 mmol·L-1L-Arg were significantly decreased(P<0.05),the S stage cell ratio increased(P<0.05),and the relative expression levels of Bax,Caspase-3,Caspase-9 mRNA and protein were significantly decreased(P<0.05),while the relative expression levels of Bcl-2 mRNA and prfotein were significantly increased(P<0.05).It is concluded that L-Arg can promote the proliferation of small intestinal epithelial cells injured by heat stress,and reduce cell apoptosis thus alleviate the damage of heat stress on intestinal epithelial cells of dairy cows in vitro. |
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| Keywords: | L-Arginine dairy cows heat stress intestinal epithelial cell apoptosis |
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