| 精密组织切片的制备及其应用 |
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| 引用本文： | 张依玲,阚子斐,牛铮,余秋寒,冉玲,张淑娟,邹宏,徐沙沙,张静怡,宋振辉.精密组织切片的制备及其应用[J].畜牧兽医学报,2022,53(2):339-348. |
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| 作者姓名： | 张依玲 阚子斐 牛铮 余秋寒 冉玲 张淑娟 邹宏 徐沙沙 张静怡 宋振辉 |
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| 作者单位： | 西南大学动物医学院, 重庆 402460 |
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| 基金项目： | 中央高校基本业务基金资助项目(XDJK2020RC001);重庆市研究生科研创新项目(CYS19137)。 |
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| 摘 要： | 精密组织切片是被研究人员广泛使用的器官体外模型,它保持了体外系统的主要优点,弥补了细胞系所缺失的功能,能充分维持组织活性、功能活性以进行毒性、诱导和感染研究.本文简要介绍了精密组织切片技术的发展进程、优点以及应用前景,对精密组织切片的制备方法、培养体系和活性测定指标,以及精密组织切片技术作为一种新型的离体模型应用于各个...
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| 关 键 词： | 精密组织切片 离体模型 药物代谢 病毒感染 致病机制 |
| 收稿时间： | 2021-06-03 |
Preparation and Application of Precision-Cut Tissue Slices |
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| ZHANG Yiling,KAN Zifei,NIU Zheng,YU Qiuhan,RAN Ling,ZHANG Shujuan,ZOU Hong,XU Shasha,ZHANG Jingyi,SONG Zhenhui.Preparation and Application of Precision-Cut Tissue Slices[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):339-348. |
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| Authors: | ZHANG Yiling KAN Zifei NIU Zheng YU Qiuhan RAN Ling ZHANG Shujuan ZOU Hong XU Shasha ZHANG Jingyi SONG Zhenhui |
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| Institution: | College of Veterinary Medicine, Southwest University, Chongqing 402460, China |
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| Abstract: | Precision-cut tissue slices are widely used by many researchers as in vitro models of organs,preserving the major advantages of in vitro systems,compensating for the lack of functionality of cell lines,and maintaining tissue activity,functional activity sufficiently for toxicity,induction and infection studies.This paper reviews the general development process,advantages and application prospects of precision tissue sectioning techniques,and reviews the preparation methods,culture systems and activity determination indexes of precision-cut tissue slices,as well as the application of precision-cut tissue slices as a novel ex vivo model for the study of various organs.It is expected to provide reference for subsequent researchers to use precision-cut tissue slices as a novel biological in vitro,ex vivo model for research. |
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| Keywords: | precision-cut tissue slices ex vivo models drug metabolism viral infection pathogenesis |
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