| 草莓根腐病病原真菌Ilyonectria novozelandica的鉴定 |
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| 引用本文： | 史芳芳,褚继萍.草莓根腐病病原真菌Ilyonectria novozelandica的鉴定[J].中国蔬菜,2022(2):95-100. |
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| 作者姓名： | 史芳芳 褚继萍 |
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| 作者单位： | 新疆生产建设兵团第十二师农业科学研究所 |
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| 基金项目： | 兵团中青年科技创新领军人才计划项目(2018CB030)。 |
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| 摘 要： | 采用形态学与分子生物学技术对草莓根腐病病原菌进行鉴定.对草莓育苗圃内发病草莓根部进行组织培养、病原菌分离纯化,通过传统的形态学观察初步判断该病原菌菌株为土赤壳属病原菌.利用通用引物ITS1/ITS4初步鉴定该病原菌菌株ZJ16属于土赤壳属,但不能鉴定到种,又选择土赤壳属特异基因EF-1α和Histone H3基因引物序...
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| 关 键 词： | 草莓根腐病 土赤壳属 多基因系统进化分析 病原鉴定 |
Identification of Strawberry Root Rot Pathogenic Fungi Ilyonectria novozelandica |
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| SHI Fangfang,CHU Jiping.Identification of Strawberry Root Rot Pathogenic Fungi Ilyonectria novozelandica[J].China Vegetables,2022(2):95-100. |
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| Authors: | SHI Fangfang CHU Jiping |
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| Institution: | (Agricultural Scientific Institute of the 12th Division of Xinjiang Production and Construction Corps,Urumchi 830088,Xinjiang,China) |
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| Abstract: | Identification on strawberry root rot pathogen was conducted by morphology and molecular biology technique.Tissue culture,pathogen isolation and purification of sicken strawberry roots in strawberry breeding nursery was carried out.The pathogen strain was initially diagnosed as Ilyonectria pathogen by traditional morphological observation.The ZJ16 strain was initially identified by universal primers ITS1/ITS4.It was belonging to the genus Ilyonectria,which could not be identified as species.The specific gene EF-1αand Histone H3 primer sequence were selected and analyzed by PCR amplification.Homology comparison between sequencing result of waiting tested strain and related strain EF-1αand Histone H3 sequence in GenBank were conducted.The homology of waiting tested strain and Ilyonectria novozelandica(CBS 112593)were all 100%.It was confirmed that strawberry root rot pathogen in Xinjiang region was identified as Ilyonectria novozelandica,which was a new recorded species in China. |
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| Keywords: | strawberry root rot Ilyonectria multigene phylogenetic analysis pathogen identification |
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