| 四川盆地6个地方鸡群体遗传关系研究 |
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| 引用本文： | 尤桂爽,易兴友,蒋美山,李中伟,谭和林,曹池,舒刚,田尧夫,朱庆,赵小玲.四川盆地6个地方鸡群体遗传关系研究[J].畜牧兽医学报,2022,53(2):370-380. |
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| 作者姓名： | 尤桂爽 易兴友 蒋美山 李中伟 谭和林 曹池 舒刚 田尧夫 朱庆 赵小玲 |
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| 作者单位： | 1. 四川农业大学动物科技学院, 成都 611130;2. 重庆市开州区农业发展服务中心, 重庆 405499 |
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| 基金项目： | 重庆市开州区农业农村委员会专项资金;2020年泸州市院士专家工作站第五批建站企业资金资助。 |
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| 摘 要： | 随着交通便利与市场化杂交利用，我国地方鸡遗传资源面临血液混杂、品种来源和品种间的遗传关系不清晰的问题。为了给第三次全国畜禽遗传资源普查工作提供依据。本研究对位于中国大巴山脉和乌蒙山区的6个地方鸡群体（每个群体公、母各10只）共118个个体进行了简化基因组GBS测序，分析了核酸多态性（Pi）、观测杂合度（Ho）、期望杂合度（He）、多态信息含量（PIC）、观测等位基因数（Na）、有效等位基因数（Ne）、遗传分化（Fst）、遗传距离（DR）和基因流（Nm）等指标。研究发现，岩水鸡黑羽群体的遗传多样性较低（He=0.154；PIC=0.125），大宁河鸡遗传多样性最为丰富（He=0.197，PIC=0.162）。城口山地鸡和大宁河鸡之间的遗传分化最低（Fst=0.053），基因流最高（Nm=4.50），丰岩乌骨鸡和岩水鸡黑羽群体之间的遗传分化最高（Fst=0.183），对应的基因流最低（Nm=1.12）。系统发生树和主成分分析显示，岩水鸡白羽群体和黑羽群体聚为一类，城口山地鸡、大宁河鸡和旧院黑鸡聚为一类，丰岩乌骨鸡单独为一类。Structure分析表明，城口山地鸡与岩水鸡白羽群体血缘混杂。本研究探明了邻近区域地方品种的遗传关系，位于乌蒙山区的丰岩乌骨鸡与大巴山脉的旧院黑鸡、城口山地鸡、大宁河鸡、岩水鸡黑羽和白羽具有较远的遗传距离。本研究为地方品种的保护和开发利用提供了依据。
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| 关 键 词： | 地方鸡品种 遗传多样性 简化基因组 基因流 系统发生树 |
| 收稿时间： | 2021-07-16 |
Study on the Genetic Relationship of Six Local Chicken Populations from Sichuan Basin |
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| YOU Guishuang,YI Xingyou,JIANG Meishan,LI Zhongwei,TAN Helin,CAO Chi,SHU Gang,TIAN Yaofu,ZHU Qing,ZHAO Xiaoling.Study on the Genetic Relationship of Six Local Chicken Populations from Sichuan Basin[J].Acta Veterinaria et Zootechnica Sinica,2022,53(2):370-380. |
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| Authors: | YOU Guishuang YI Xingyou JIANG Meishan LI Zhongwei TAN Helin CAO Chi SHU Gang TIAN Yaofu ZHU Qing ZHAO Xiaoling |
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| Institution: | 1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;2. Agricultural Development Service Center of Kaizhou District, Chongqing 405499, China |
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| Abstract: | With the convenience of transportation and market-oriented cross utilization,the genetic resources of local chickens in China are facing the problems of blood mixing and the unclear breed source and genetic relationship among breeds.The study aimed to provide a basis for the Third National Census of Livestock and Poultry Genetic Resources.A simplified genomic GBS sequencing was carried out on 118 individuals from 6 local chicken populations(10 males and 10 females in each population)located in Daba Mountains and Wumeng Mountains in China.The nucleotide polymorphism(Pi),observed heterozygosity(Ho),expected heterozygosity(He),polymorphic information content(PIC),number of observed alleles(Na),number of effective alleles(Ne),genetic differentiation(Fst),genetic distance(DR)and gene flow(Nm)of these populations were analyzed.It was found that the genetic diversity of black feather population of Yanshui chicken was low(He=0.154;PIC=0.125),and the genetic diversity of Daninghe chicken was the most abundant(He=0.197,PIC=0.162).The genetic differentiation between Chengkou mountain chicken and Daninghe chicken was the lowest(Fst=0.053),the gene flow was the highest(Nm=4.50),the genetic differentiation between Fengyan black bone chicken and black feather population of Yanshui chicken was the highest(Fst=0.183),and the corresponding gene flow was the lowest(Nm=1.12).Phylogenetic tree and principal component analysis showed that the white feather population and black feather population of Yanshui chicken were clustered into one class.Chengkou mountain chicken,Daninghe chicken and Jiuyuan black chicken were clustered into one class,while Fengyan black bone chicken was a separate class.Structure analysis showed that the blood of Chengkou mountain chicken and white feather population of Yanshui chicken was mixed.This study proved the genetic relationship of local breeds in adjacent areas.Fengyan black bone chicken located in Wumeng Mountain has a long genetic distance from Jiuyuan black chicken,Chengkou mountain chicken,Daninghe chicken,black feather and white feather populations of Yanshui chicken in Daba Mountain.This study provides a basis for the protection,development and utilization of local chicken breeds. |
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| Keywords: | local chicken breeds genetic diversity simplified genome gene flow phylogenetic tree |
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