| 相关序列扩增多态性(SRAP)一种新的分子标记技术 |
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| 作者姓名： | 任 羽 王得元 张银东 |
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| 作者单位： | 热带作物生物技术国家重点实验室，广东省农业科学院蔬菜研究所/广东省果蔬新技术研究重点实验室，热带作物生物技术国家重点实验室 海南海口571101广东省农业科学院蔬菜研究所/广东省果蔬新技术研究重点实验室，广州510640，广州510640，海南海口571101 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(2002AA207012-2);国家自然科学基金项目:广东省重点科技攻关项目(A20202、2002A2070201);广东省自然科学基金(000162);广州市重点科技攻关项目(2002Z2-E0192);广东省农业科学院院长基金项目(2001-基金-08、04-基金-03B). |
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| 摘 要： | SRAP是一种新的DNA分子标记,具有简便、稳定、中等产率和容易得到选择条带序列的特点。SRAP是一种基于PCR技术的新的分子标记技术,其利用独特的引物设计对ORFs进行扩增,上游引物长17bp,对外显子进行特异扩增,下游引物长18bp,对内含子区域、启动子区域进行特异扩增,因个体不同以及物种的内含子、启动子与间隔长度不等而产生多态性。扩增产物用变性的聚丙烯酰胺凝胶分离,然后用放射自显影检测。本文在阐述SRAP的原理和流程后,详细论述了SRAP标记目前在植物遗传图谱构建、遗传多样性、基因定位、比较基因组学、杂种优势预测等方面的研究进展及应用前景。
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| 关 键 词： | SRAP 分子标记 应用 |
Sequence-related Amplified Polymorphism(SRAP):A Novel Technique for Molecular Marker |
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| Authors: | Ren Yu Wang Deyuan Zhang Yindong |
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| Institution: | (1Vegetable Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Biotech Lab for Fruits and Vegetables, Guangzhou 510640;2National Key Biotech Lab for Tropical Crop, Haikou 571101) |
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| Abstract: | SRAP is a new technique for molecular marker .It is simplicity, reliability, moderate throughput ratio and facile sequencing of selected bands. This paper describes the SRAP principle, protocol. SRAP aimed for amplification of open reading frames(ORFs) by special primer set. The forward prime of 17 base amplified exons, the reverse primer of 18 base amplified introns and promoters. Since introns, promoters and spacers are variable among different individuals, this makes it feasible to generate polymorphism. The amplified DNA fragments are separated by denaturing acrylamide gels and detected by autoradiography. This technique has been used in genetics mapping, genetic diversity, gene tagging, comparative genomics and heterosis prediction. The prospect of the SRAP marker application in plant genetics and research were discussed. |
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| Keywords: | Sequence-related Amplified Polymorphism Molecular marker application |
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