| 根癌农杆菌介导玉米遗传转化的研究进展 |
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| 作者姓名： | 高武军 卢龙斗 魏开发 孙富丛 |
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| 作者单位： | 河南师范大学生命科学院 河南新乡453002
(高武军，卢龙斗，魏开发)，河南师范大学生命科学院 河南新乡453002(孙富丛) |
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| 基金项目： | 河南师范大学青年科学基金(20040011)，河南师范大学青年骨干教师基金资助 |
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| 摘 要： | 对根癌农杆菌介导的玉米基因转化中包括外植体、基本培养基及其附加物、共培养温度、信号分子与vir基因的活化的几个关键因素研究进展作了论述。近年来的研究发现,大小为1~2mm的幼胚是较为理想的转化外植体,在外植体培养基本培养基一般多选用MS和N6,在基本培养基上附加有机氮和氨基酸、金属离子均有利于转化效率的提高。转化中的共培养温度一般在200C~250C之间。研究认为根癌农杆菌转化禾本科植物的困难在于禾本科植物缺乏酚类物质,难以诱导Vir基因活化表达,因此在转化中多采用加入外源的酚类物质如乙酰丁香酮来提高玉米转化的成功率。
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| 关 键 词： | 玉米 根癌农杆菌 基因转化 |
Research Progress of Gene Transformation in Maize using Agrobacterium tumefaciens |
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| Authors: | Gao wujun Lu longdou Wei kaif Sun fucong Li ruili |
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| Institution: | (College of Life Science ,Henan Normal university, Xinxiang 453002) |
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| Abstract: | Researching progress of several key factors that maize gene transformation using Agrobacterium tumefaciens were reviewed,including explant,base-medium and supplementary, co-culture temperature,signal molecular and Vir gene activation?Research found that immature embryos are the optimical explants especially when it is from 1mm to 2mm , the co-culture temperature from 200C to 250C. Research demonstrate that the barrier of transfer the foreign gene to the gramineous plants using Agrobacterium tumefaciens is lack phenol and it is difficult to induce the Vir gene activity.So supplementary the acetosyringon in co-culture medium was essential to efficient transfer foreign gene to gramineous plants. Besides, a high incidence of T-DNA transfer was also closely associated to the supplementary such as organic nitrogen , amino acid and metal ions. |
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| Keywords: | Maize Agrobacterium tumefaciens Gene transormation |
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