| 影响长寿花离体培养及植株再生的几个因素 |
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| 作者姓名： | 黄海帆 李保印 李 平 |
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| 作者单位： | 河南农业大学农业职业学院园艺系 郑州451450
(黄海帆)，河南职业技术师范学院 新乡453003
(李保印)，河南农业大学农业职业学院园艺系 郑州451450(李平) |
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| 摘 要： | 对长寿花茎段培养 7～ 8d ,部分茎段腋芽萌动。 30d培养结果表明 ,芽诱导的最佳培养基是 :MS 6 -BA1 0mg/L NAA0 5mg/L ;芽伸长的最佳培养基是 :1/ 4MS ;形成有效节的最佳培养基是 :MS。继代培养中各培养基上无叶茎段均较带叶茎段萌芽率高。在 5、 6、 7号培养基上 ,叶片培养 9d时 ,从叶柄基部开始形成不定芽 ,在其它培养基上 ,14d才开始有不定芽形成。 30d培养结果还表明 ,诱导叶片再生不定芽的最佳培养基是 :1/ 2MS 6 -BA1 0mg/L。诱导根生长的最佳培养基是 :1/ 4MS
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| 关 键 词： | 长寿花(Kalanchoe bloss feldiana) 离体培养及植株再生 影响因素 |
Several Factors Influencing in Vitro Culture and Plantlet Regeneration of Kalanchoe blossfediana |
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| Authors: | Huang Haifan Li Baoyin Li Ping |
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| Institution: | (1. Agricultural Vocational College, Henan Agricultural University, Zhengzhou 451450
2.Henan Vocation-technical Teachers College, Xinxiang 453003) |
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| Abstract: | Having cultured the stem sections of Kalanchoe blossfeldiana in vitro for7-8days, the axillary buds of explants can germinate. The result of culturing for 30 days showed that the most suitable medium to induce buds was MS+6-BA1.0+NAA0.5 mg?l-1, the most suitable one to make the bud stretch was 1/4MS, and to form available knots was MS. During the continuing culture, the germinating rate of stem sections with no leaves was higher than that of the sections with leaves on all the above media. Adventitious buds began to form at the base of petiole after having been cultured for 9 days on the media No.5,6,7. On the other media, however, they only began to form after being cultured for 14days. Simultaneously, the most suitable medium to induce the leaves to regenerate adventitious buds was 1/2MS+6-BA1.0 mg?l-1, and to induce the roots to grow was 1/4MS. |
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| Keywords: | Kalanchoe blossfeldiana Vitro culture and plantlet regeneration Influence factors |
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