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Canine pre-iridal fibrovascular membranes: morphologic and immunohistochemical investigations |
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| Authors: | Mitzi K Zarfoss Carrie B Breaux† Herbert E Whiteley Ralph E Hamor Jodi A Flaws‡ Philippe Labelle§ Richard R Dubielzig¶ |
| Institution: | Resident in Comparative Ophthalmology, University of Illinois, College of Veterinary Medicine, Small Animal Clinic, 1008 W. Hazelwood Drive, Urbana, IL 61802, USA;, WestVet Emergency and Specialty Center, Ophthalmology, Boise, ID 83714, USA;, University of Illinois at Urbana-Champaign, Department of Veterinary Biosciences, Urbana, IL 61802, USA;, Antech Diagnostics, Pathology, Lake Success, NY 11042, USA;, University of Wisconsin, Department of Pathobiological Sciences, Madison, WI 53706, USA |
| Abstract: | Objective Pathologic intraocular neovascularization is a key component of many canine ophthalmic diseases such as uveitis, retinal detachment, intraocular neoplasms, and corneal perforation. The purpose of this study was to evaluate the structure of pre-iridal fibrovascular membranes (PIFMs) associated with several different disease processes and to identify specific factors associated with their development in the canine eye. Procedure This study examined 36 enucleated canine eyes with the diagnosis of PIFM and one of the following: lens-induced uveitis, retinal detachment, iridociliary adenoma, corneal perforation, severe hyphema, or vitreal gliovascular membranes (canine ocular gliovascular syndrome, COGS). Three histologic stains and six immunohistochemical stains were performed in all 36 PIFM eyes and four histologically normal eyes, including: hematoxylin and eosin, alcian blue periodic acid schiff (PAS), Masson's trichrome, platelet endothelial cell adhesion molecule-1 (CD31), smooth muscle actin, vimentin, laminin, vascular endothelial growth factor (VEGF), and cyclooxygenase-2 (COX-2). Results Pre-iridal fibrovascular membrane extracellular matrix staining was consistent with collagen and mucins in all cases and positive for laminin in most cases. All PIFMs contained CD31-positive vessels and predominantly lymphoplasmacytic inflammation. Both PIFM vessels and spindle cells were positive for laminin, vimentin, smooth muscle actin, VEGF, and COX-2. Secondary intraocular pathology and immunohistochemical staining of other intraocular structures are also reported. Conclusions Pre-iridal fibrovascular membrane morphology and immunohistochemical characteristics were similar across six canine disease processes, suggesting analogous pathophysiologic mechanisms. COX-2 and VEGF were identified using immunohistochemistry and may play a role in PIFM development. |
| Keywords: | angiogenesis canine eye immunohistochemistry inflammation iris |