| 金钗石斛菌根、茎和叶内生真菌的分离与鉴定 |
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| 引用本文： | 黎勇,罗培凤,朱丹丽,齐泽民.金钗石斛菌根、茎和叶内生真菌的分离与鉴定[J].安徽农业科学,2012,40(23):11598-11600,11618. |
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| 作者姓名： | 黎勇 罗培凤 朱丹丽 齐泽民 |
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| 作者单位： | 内江师范学院生命科学学院,四川内江,641000;内江师范学院生命科学学院,四川内江,641000;内江师范学院生命科学学院,四川内江,641000;内江师范学院生命科学学院,四川内江,641000 |
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| 摘 要： | 目的]分离鉴定铁皮石斛不同组织内生真菌,以期为提高其组培苗的大田定植成活率提供试验依据。方法]以改良PDA-抗生素酸性培养基为基础分离培养基,分别从金钗石斛菌根、茎和叶中分离内生真菌,利用切片法对分离得到的菌株进行纯化,并观察其菌落形态。结果]通过菌落形态观察,初步鉴定所纯化的6个内生菌株分别为:NJJG-1,NJJG-2,NJJR-1,NJJR-2,NJJR-3和NJYJ-1。试验结果表明材料表面消毒条件和方法以及分离培养基的选用,均直接影响真菌的分离纯化效果和分离得到的内生菌的多样性,其中以75%酒精处理30 s,再用0.1%升汞处理7 min的表面消毒效果稳定,再配合改良的PDA-抗生素酸性培养基分离内生菌,可以分离出较多的内生菌种类。结论]该研究结果为铁皮石斛不同组织内生真菌的分离鉴定提供了指导方法,同时为提高其组培苗的大田定植成活率提供了试验依据。
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| 关 键 词： | 金钗石斛 铁皮石斛 内生真菌 |
Isolation and Identification of Endophytic Fungi in the Root, Stem and Leaves of Dendrobium nobile Lindl |
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| Institution: | LI Yong et al(College of Life Sciences,Neijiang Normal University,Neijiang,Sichuan 641000) |
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| Abstract: | Objective] To isolate and identify the endophytic fungi in different tissues of Dendrobium nobile Lindl.,so as to provide experimental basis for its plants survival rate.Method] The endophytic fungi was isolated from the root,stem and leaves of Dendrobium nobile Lindl.on the improved PDA-antiobiotic acidic medium,and the isolated strains were purified by the slicing method,and their colony morphology was observed.Result] Through the observation of colony morphology,the six endophytic fungi were identified as NJJG-1,NJJG-2,NJJR-1,NJJR-2,NJJR-3,NJYJ-1,respectively.The experimental results indicated that the purification results and diversity of endophytic fungi were directly influenced by the surface disinfection conditions and methods of materials and the screening of separating medium.The treatment effect of 75% alcohol for 30 seconds plus 0.1% mercuric chloride for 7 minutes was stable,and more species of endophytic fungi were isolated combined with the improved PDA-antiobiotic acidic medium.Conclusion] The study provided a guidance for the isolation and identification of endophytic fungi in different tissues of Dendrobium nobile Lindl.,and provided experimental basis for the survival rate of its field plants. |
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| Keywords: | Dendrobium nobile Lindl Dendrobium Officinale Endophytic fungi |
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