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菱角壳粗多糖体外清除自由基活性的研究
引用本文:刘志国,赵文亚.菱角壳粗多糖体外清除自由基活性的研究[J].安徽农业科学,2012,40(22):11182-11183,11209.
作者姓名:刘志国  赵文亚
作者单位:枣庄学院体育学院,山东枣庄,277160;枣庄学院生命科学学院,山东枣庄,277160
摘    要:目的]研究菱角壳粗多糖体外清除自由基的活性。方法]采用水提醇沉法从菱角壳中提取粗多糖,并对菱角壳粗多糖在体外清除羟自由基(.OH)、1,1-二苯基苦基苯肼(DPPH.)和超氧阴离子(O2-.)的能力进行了研究。结果]随着浓度的增加,菱角壳粗多糖对3种自由基的清除能力均呈现增强的趋势,在多糖浓度为2.5 mg/ml时,菱角壳粗多糖对羟自由基、DPPH和超氧阴离子的清除率分别为70.6%、66.2%和48.6%。结论]菱角壳粗多糖具有较强的清除自由基的能力。

关 键 词:菱角壳  粗多糖  自由基  体外

Study on in vitro Free-radicals Scavenging Activity of the Coarse Polysaccharide from the Hull of Water Chestnut
Institution:LIU Zhi-guo et al(Physical Education Institute,Zaozhuang University,Zaozhuang,Shandong 277160)
Abstract:Objective]The aim was to study the in vitro free-radicals scavenging activity of the coarse polysaccharide from the hull of water chestnut.Method]The coarse polysaccharide from the hull of water chestnut was extracted by water extraction and alcohol precipitation.The abilities of the coarse polysaccharide from the hull of water chestnut to scavenge ·OH,DPPH· and O2-· were evaluated.Results]The scavenging rate of the coarse polysaccharide from the hull of water chestnut on ·OH,DPPH· and O2-· was enhanced with the increment of polysaccharide concentration.When the polysaccharide concentration was 2.5 mg/ml,the scavenging rate of the coarse polysaccharide from the hull of water chestnut on ·OH,DPPH· and O2-· were 70.6%,66.2% and 48.6%.Conclusion] The coarse polysaccharide from the hull of water chestnut had significant scavenging activity on free radicals.
Keywords:Hull of water chestnut  Coarse polysaccharide  Free-radicals  In vitro
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