| 鲤鱼肝细胞的原代培养研究 |
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| 引用本文： | 李月红,吉尚雷,吴东明,谭崇桂.鲤鱼肝细胞的原代培养研究[J].安徽农业科学,2012,40(22):11278-11279. |
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| 作者姓名： | 李月红 吉尚雷 吴东明 谭崇桂 |
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| 作者单位： | 吉林农业大学动物科技学院,吉林长春,130118;吉林农业大学动物科技学院,吉林长春,130118;吉林农业大学动物科技学院,吉林长春,130118;吉林农业大学动物科技学院,吉林长春,130118 |
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| 基金项目： | 国家自然科学基金，吉林省科技发展计划资助项目 |
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| 摘 要： | 目的]探讨鲤鱼肝细胞原代培养的最佳条件。方法]通过对鲤鱼的肝脏细胞进行原代培养,分析不同培养基、温度、pH、胰蛋白酶浓度、生长时间对鲤鱼肝细胞生长状况的影响,并测定肝细胞活力。结果]鲤鱼肝细胞培养的最佳条件为:温度在25℃左右,pH7.4,胰蛋白酶浓度0.250%,消化时间40 min,培养基为M199。分离肝细胞的平均存活率>85%,每克肝平均可获得3.8×106个分离细胞,在细胞活力及数量上达到最佳平衡。结论]该研究可为建立稳定的毒理学和药理学试验模型奠定基础。
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| 关 键 词： | 鲤鱼 肝细胞 原代培养 |
Study on the Primary Culture of Hepatocytes from Common Carp |
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| Institution: | LI Yue-hong et al(College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Objective] The research aimed to discuss the best conditions for the primary culture of hepatocyte cells from common carp.Method] The primary culture of hepatocytes from common carp was conducted to analyze the effects of different medium,temperature,pH,trypsinase concentration,growing time on the growth situations of hepatocytes from common carp.Result] The optimum culture conditions of hepatocyte cells from common carp were as follows:temperature of about 25 ℃,pH of 7.4,trypsinase concentration of 0.250%,digestion time of 40 min,culture medium M199.The average survival rate of the isolated hepatocytes was above 85%.3.8×106 isolated cells could be obtained per gram of liver,and the best balance between the cell viability and quantity could be achieved.Conclusion] The research could provide basis for establishing stable toxicological and pharmacological experiment models. |
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| Keywords: | Common carp Hepatocyte Primary culture |
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