| 香椿蛋白质对小鼠抗氧化能力及非特异免疫的影响 |
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| 引用本文： | 张林甦,夏亚兰,彭泽萍.香椿蛋白质对小鼠抗氧化能力及非特异免疫的影响[J].安徽农业科学,2012,40(21):10840-10841,10856. |
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| 作者姓名： | 张林甦 夏亚兰 彭泽萍 |
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| 作者单位： | 黔南民族医学高等专科学校,贵州都匀558003;贵州大学贵州省农业生物工程重点实验室,贵州贵阳550025;黔南民族医学高等专科学校,贵州都匀,558003 |
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| 基金项目： | 贵州省教育厅自然科学研究项目，贵州省卫生厅基金，黔南民族医学高等专科学校科研基金 |
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| 摘 要： | 目的]研究香椿蛋白质对小鼠抗氧化能力及非特异免疫的影响。方法]用硫酸铵沉淀法、透析、冷冻干燥的方法提取香椿蛋白粗品,然后分别以高、中、低剂量的香椿蛋白腹腔注射小鼠9 d,取一半数量小鼠的血清,用试剂盒测定血清的SOD和GSH-Px,并解剖出肝脏、脾脏和胸腺,测定丙二醛MDA含量及胸腺和脾脏指数;另一半小鼠于第10天腹腔注射淀粉肉汤,然后取腹腔液观察巨噬细胞的吞噬率。结果]香椿粗品蛋白的含量为84.5%,得率为5.3%。香椿蛋白可明显提高小鼠血清SOD的活性(P<0.05,高剂量P<0.01);高剂量香椿蛋白可明显增加GSH-Px的含量(P<0.05)、脾脏指数(P<0.05,高剂量P<0.01)和巨噬细胞的吞噬率(P<0.05)。结论]香椿蛋白具有一定的体内抗氧化能力,对小鼠非特异性免疫也有一定的促进作用。
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| 关 键 词： | 香椿蛋白 小鼠 体内抗氧化 非特异免疫 |
Effects of Toona sinensis Protein on the Antioxidant Capacity and Nonspecific Immunity of Mice |
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| Institution: | ZHANG Lin-su et al(Qiannan Medical College for Nationalities,Dunyun,Guizhou 558003) |
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| Abstract: | Objective] To study the effects of Toona sinensis protein on the antioxidant capacity and nonspecific immunity of mice.Method] Crude protein was extracted from T.sinensis by dialysis,freeze drying,and ammonium sulphate precipitation.Mice were given T.sinensis protein by intraperitoneal injection for 9 d in high-,middle-and low-dosages.Serums of half of mice were selected and their SOD and GSH-Px were detected by kits.Liver,spleen,and thymus were anatomized.MDA content and spleen and thymus indices were detected.The other half mice were given starch broth at 10 d by intraperitoneal injection.Then,peritoneal fluid was taken out and the phagocytic rate of macrophage was observed.Result] Content of T.sinensis crude protein was 84.5% and the extraction rate was 5.3%.T.sinensis protein could significantly enhance the activity of SOD in mice serum(P<0.05,high dosage P<0.01).High-dosage T.sinensis protein could enhance the content of GSH-Px(P<0.05),spleen index(P<0.05,high dosage P<0.01),and phagocytic rate of macrophage(P<0.05).Conclusion] T.sinensis protein had certain in vivo antioxidant capacity,and could promote the nonspecific immunity of mice. |
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| Keywords: | Toona sinensis protein Mice Antioxdation in vivo Nonspecific immunity |
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