| 草地早熟禾愈伤组织诱导及植株再生 |
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| 引用本文： | 信金娜,韩烈保,刘君,罗莉,李雪.草地早熟禾愈伤组织诱导及植株再生[J].中国草地学报,2004,26(4):46-50. |
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| 作者姓名： | 信金娜 韩烈保 刘君 罗莉 李雪 |
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| 作者单位： | 北京林业大学草坪研究所,北京,100083 |
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| 基金项目： | 国家转基因植物研究与产业化专项项目(J2002-B-006)，国家863项目(2001AA244082)资助 |
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| 摘 要： | 以成熟种子和胚轴为外植体诱导草地早熟禾愈伤组织,比较草地早熟禾四个品种的愈伤诱导情况和不同6-BA浓度、愈伤年龄等因素对愈伤组织分化能力的影响。结果表明:成熟种子的出愈率与胚性愈伤诱导率均高于胚轴;MS 2,4—D(2mg/L) 6-BA(0.1mg/L)培养基为草地早熟禾Mardona品种较为合适的愈伤培养基,其诱导率为58.3%;MS BA(3mg/L) KT(0.2mg/L)为较为适合的分化培养基,再生率高达70%;随着继代次数的增加,草地早熟禾分化能力能够继续保持。选择致密、易碎、生长迅速的愈伤继代能够保持草地早熟禾的胚性,可以为遗传转化提供长期良好的植物材料。
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| 关 键 词： | 草地早熟禾 愈伤 分化 |
| 文章编号： | 1000-6311(2004)04-0046-05 |
| 修稿时间： | 2003年12月31 |
Study on Callus Induction and Plant Regeneration System of Poa pratensis |
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| XIN Jin-na,HAN Lie-bao,LIU Jun,LUO Li,LI Xue.Study on Callus Induction and Plant Regeneration System of Poa pratensis[J].Chinese Journal of Grassland,2004,26(4):46-50. |
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| Authors: | XIN Jin-na HAN Lie-bao LIU Jun LUO Li LI Xue |
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| Abstract: | The tissue culture responses of mature seeds and radicel were compared.Callus induction were studied in four cultivars of Poa pratensis L.Effect of 6-BA concentration,callus_age and other factors on differentiation ability were studied.Results showed that mature seed as explants had higher appearance rate than radicel;MS medium,supplemented with 2mg/L 2,4-D and 0.1mg/L BA was the best medium for cultivar'Mardona'of Poa pratensis L.,callus induction rate was 58.3%;MS medium,supplemented with 3mg/L BA and 0.2 mg/L KT was suitable for differentiation medium,regeneration rate was 70%; with subculture times increased,it still keep differentiation ability.Selecting compact,friable and grown rapidly callus subculture,can always provided good plant material for gene transfer for long term. |
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| Keywords: | Poa pratensis L Callus Differentiations |
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