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| 药用植物台湾金线莲快繁技术研究 | |
| 引用本文： | 孔琼,袁盛勇,张庭香,查应洪,张薇.药用植物台湾金线莲快繁技术研究[J].安徽农业科学,2008,36(6):2272-2273. |
| 作者姓名： | 孔琼 袁盛勇 张庭香 查应洪 张薇 |
| 作者单位： | 1. 云南红河学院生物系,云南蒙自,661100 2. 云南红河学院农学系,云南蒙自,661100 3. 云南农业大学园林园艺学院,云南昆明,650201 4. 云南省屏边县组培育苗有限公司,云南屏边,661200 |
| 摘 要： | 目的]为台湾金线莲的深入研究提供种源。方法]以带腋芽的台湾金线莲茎段为外植体,用不同培养基诱导丛生芽,并进行生根诱导,建立一套快速繁殖体系。结果]单独加入1.0~4.0 mg/L6-BA的培养基使台湾金线莲丛生芽的增殖倍数从2.3增至4.5;同时添加6-BA和2,4-D时,丛生芽的增殖倍数明显增加,6-BA和2,4-D浓度分别为2.0、0.4 mg/L时,丛生芽的增殖倍数达8.1。1/10 MS培养基较适宜生根诱导。活性炭浓度小于0.1%有利于组培苗的生根及生长,生根所需时间较短,平均生根数有所增加,植株生长健壮,经过10~20 d的炼苗,移栽成活率达95%以上。结论]台湾金线莲丛生芽诱导的适宜培养基为MS+1.0 mg/L KT+0.2 mg/L NAA+2.0mg/L6-BA+0.4 mg/L2,4-D,生根诱导的适宜培养基为1/10 MS+0.5 mg/L NAA+5%香蕉泥+0.05%活性炭。 |
| 关 键 词： | 金线莲 丛生芽 生根诱导 |
| 文章编号： | 0517-6611(2008)06-02272-01 |
| 修稿时间： | 2007年10月30 |
Rapid Propagation of Medical Plant Taiwan Anoectochilus formosanus |
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| KONG Qiong.Rapid Propagation of Medical Plant Taiwan Anoectochilus formosanus[J].Journal of Anhui Agricultural Sciences,2008,36(6):2272-2273. | |
| Authors: | KONG Qiong |
| Abstract: | Objective] The study aimed to supply seed sources for deeply researching Taiwan Anoectochilus formosanus.Method] With stem segments having axillary buds of Taiwan A.formosanus as explants,the different media were used to induce clustered buds and conduct rooting induction so as to establish a set of rapid propagation system.Result] The media added 1.0～4.0 mg/L 6-BA solely increased the proliferation multiple of A.formosanus clustered buds from 2.3 to 4.5 times.When 6-BA and 2,4-D were added at the same,the proliferation multiple of clustered buds increased obviously.When the concentrations of 6-BA and 2,4-D were 2.0 and 0.4 mg/L resp.,the proliferation multiple of clustered buds reached 8.1.1/10 MS medium was more suitable for rooting induction.The active carbon concentration smaller than 0.1% was favorable for the rooting and growth of tissue cultured seedlings,the time needed for taking root was shorter and the average rooting number had some increment,the plant grew healthily and the transplant survival rate was over 95% after seedling-hardening for 10～20 d.Conclusion] The optimum medium was MS+1.0 mg/L KT +0.2 mg/L NAA +2.0 mg/L 6-BA+0.4 mg/L 2,4-D for inducing clustered buds of Taiwan A.formosanus and was 1/10 MS+0.5 mg/L NAA+5% banana juice+0.05% active carbon for their rooting inductions. |
| Keywords: | Anoectochilus formosanus Clustered buds Rooting induction |
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