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Isolation and characterization of microorganisms capable of hydrolysing the herbicide mefenacet |
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| Authors: | Naoki Harada Kazuhiro Takagi Kunihiko Fujii |
| Institution: | a Kowa Research Institute, Kowa Co., Ibaraki 305-0856, Japan b National Institute for Agro-Environmental Sciences, Ibaraki 305-0856, Japan c i-CUBE Tsukuba, Ibaraki 305-0032, Japan d WDB, Tokyo 100-0004, Japan e Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan |
| Abstract: | Three strains—Nocardioides sp. MFC-A, Rhodococcus rhodochrous MFC-B, and Stenotrophomonas sp. MFC-C—were newly isolated from rice soil or bark compost as microorganisms capable of cometabolically degrading mefenacet (2-benzothiazol-2-yloxy-N-methyl-acetanilide). Several other species belonging to the genera Nocardioides, Rhodococcus, and Stenotrophomonas were also capable of degrading mefenacet, suggesting that the ability is not specific to these isolates, but common at least in these genera. N-methylaniline and 2-benzothiazoloxy acetic acid were identified as the metabolites by liquid chromatography-mass spectrometry. Besides mefenacet, the isolates could degrade one or more other amide pesticides, such as flutolanil, mepronil, metolachlor, and pretilachlor. These results indicate that the strains Nocardioides sp. MFC-A, Rhodococcus rhodochrous MFC-B, and Stenotrophomonas sp. MFC-C degrade mefenacet via hydrolysis of the amide bond, but their substrate specificities differ. |
| Keywords: | Hydrolysis Isolation Liquid chromatography-mass spectrometry Mefenacet Nocardioides Rhodococcus Stenotrophomonas |
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