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Phylogeny and nodulation signal molecule of rhizobial populations able to nodulate common beans—other than the predominant species Rhizobium etli—present in soils from the northwest of Argentina |
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| Authors: | O Mario Aguilar M Verónica López Belén Morón Clemente Mateos Carolina Sousa |
| Institution: | a Instituto de Bioquímica y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, (1900) La Plata, Argentina b Departamento de Química Orgánica, Facultad de Química, Universidad de Sevilla, 41071 Sevilla, Spain c Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Sevilla, 41071 Sevilla, Spain d Laboratorio de Sistemática y Biología Evolutiva, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Paseo del Bosque s/n, (1900) La Plata, Argentina |
| Abstract: | We examined the bean rhizobia community other than the predominant species Rhizobium etli present in soils of a region that is part of the range occupied by the host in Northwest Argentina, which showed Rep and 16S rDNA RFLP polymorphism. Two populations represented by isolates T29N3L and T44N22P were found to be distinct chromosomal genotypes and closely related to species Rhizobium tropici and Agrobacterium rhizogenes. Their symbiotic genes were analyzed and found to cluster with those from R. tropici as well as with rhizobia isolated from leguminous trees. Three nodulation metabolites produced by T44N22P were detected which are tetra- and pentameric chitocompounds, N-methylated, O-carbamoylated, and N-substituted either by a C18:0 or C18:1 acyl chain at their non-reducing end, and all them sulphated at the reducing end. Isolates T29N3L and T44N22P exhibited broad host range but unlike T29N3L, only T44N22P was able to efficiently nodulate Medicago truncatula. |
| Keywords: | Bean rhizobia ecology Rhizobial diversity Symbiotic genes nodC nodH and nifH Nodulation factor structure |
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