| 抗黑曲霉乳酸菌筛选及其抑菌性能研究 |
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| 引用本文： | 陈晖,杨晓聪,张玲玲,陈龙,朱静.抗黑曲霉乳酸菌筛选及其抑菌性能研究[J].现代农业科技,2023(16):192-195+199. |
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| 作者姓名： | 陈晖 杨晓聪 张玲玲 陈龙 朱静 |
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| 作者单位： | 信阳农林学院 食品学院,信阳农林学院 食品学院,信阳农林学院 食品学院,信阳农林学院 食品学院,信阳农林学院 食品学院 |
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| 基金项目： | 信阳农林学院高水平科研孵化器建设(FCL202014)；信阳农林学院科研促进教学专项课题(kj-2022015)。 |
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| 摘 要： | 本文以10株小米发糕源乳酸菌为研究对象，采用双层平板划线法和96孔板法筛选出对黑曲霉具有较好抑制效果的乳酸菌株，并以乳酸菌对黑曲霉菌落的抑菌率为指标，研究不同pH值、温度、酶处理下乳酸菌对黑曲霉的抑制效果。结果表明：菌株1-6-2X抗黑曲霉效果最好，抑菌率达到87.78%，在pH值3.0～5.0时仍保持抑菌性，有良好的热稳定性，经胃蛋白酶、胰蛋白酶、中性蛋白酶处理后抑菌率显著下降，可作为开发抗黑曲霉乳酸菌的出发菌株。
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| 关 键 词： | 乳酸菌 黑曲霉 筛选 抑菌性能 |
| 收稿时间： | 2022/12/9 0:00:00 |
| 修稿时间： | 2022/12/9 0:00:00 |
Screening of Lactic Acid Bacteria Inhibiting Aspergillus niger and Its Antibacterial Activity |
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| Abstract: | In this paper, 10 strains of lactic acid bacteria isolated from millet cake were selected as the research object, and lactic acid bacteria with good inhibition effect on Aspergillus niger were screened by double plate scribing method and 96 well plate method. The inhibition effect of lactic acid bacteria on Aspergillus niger colony was studied under different pH, heating temperature, protease and catalase treatment conditions, with the inhibition rate of lactic acid bacteria on Aspergillus niger colony as the index. The results showed that the strain 1-6-2X had the best inhibitory effect on Aspergillus niger, reaching 87.78%. It remained bacteriostatic at pH 3.0~5.0 and had good thermal stability. After treatment with pepsin, trypsin and neutral protease, the bacteriostatic activity decreased significantly, which can be used as the starting strain for developing active lactic acid bacteria against Aspergillus niger. |
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| Keywords: | inhibition of Aspergillus niger lactic acid bacteria screening bacteriostatic property |
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