| 白及花花青素微波提取方法的优化 |
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| 引用本文： | 王祯,王洁,项海萍,樊泽鹏,孙诚蔓,邢丙聪,邵清松.白及花花青素微波提取方法的优化[J].浙江农林大学学报,2020,37(5):1020-1026. |
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| 作者姓名： | 王祯 王洁 项海萍 樊泽鹏 孙诚蔓 邢丙聪 邵清松 |
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| 作者单位： | 1.浙江农林大学 亚热带森林培育国家重点实验室，浙江 杭州 3113002.浙江农林大学 林业与生物技术学院，浙江 杭州 311300 |
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| 基金项目： | 浙江省农业新品种选育重大科技专项(2016C02058)；国家级大学生创新创业训练计划项目(201910341059X) |
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| 摘 要： | 目的 采用响应面法对白及Bletilla striata花中花青素的提取方法进行优化。 方法 采用微波萃取和pH示差法提取和测定白及花中花青素，在通过单因素法获得微波功率、甲醇体积分数、提取时间和料液比4个单因素最佳参数的基础上，利用响应面分析法建立二次回归方程，优化花青素最佳提取参数。 结果 所得优化模型条件与实际提取拟合度良好。pH示差法调整为:测定波长530 nm，溶液平衡时间80 min。本研究范围内，4个因素对提取量影响从大到小顺序为微波功率、料液比、甲醇体积分数、微波时间。在微波功率640 W，甲醇体积分数90%，微波时间22 min和料(g)液(mL)比1∶48的条件下，白及花中总花青素提取量为6.68 mg·g?1，与理论预测值6.83 mg·g?1基本一致。 结论 优化所得方法可用作白及花中花青素的提取制备，为日后白及花的综合开发利用提供依据。图8表2参22
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| 关 键 词： | 白及花 花青素 pH示差法 微波萃取 响应面 |
| 收稿时间： | 2019-10-08 |
Optimization of microwave-assisted extraction of anthocyanins from Bletilla striata flowers |
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| WANG Zhen,WANG Jie,XIANG Haiping,FAN Zepeng,SUN Chengman,XING Bingcong,SHAO Qingsong.Optimization of microwave-assisted extraction of anthocyanins from Bletilla striata flowers[J].Journal of Zhejiang A&F University,2020,37(5):1020-1026. |
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| Authors: | WANG Zhen WANG Jie XIANG Haiping FAN Zepeng SUN Chengman XING Bingcong SHAO Qingsong |
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| Institution: | 1.State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China2.College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China |
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| Abstract: | Objective This study is aimed at the optimization of microwave-assisted extraction of anthocyanins from Bletilla striata flowers. Method With anthocyanins in B. striata flowers determined and extracted via microwave-assisted extraction and pH Differential Method and the optimum parameters of the four single factors of microwave power, methanol concentration, extraction time and solid-liquid ratio obtained, the study has managed to obtain the optimal extraction parameters of anthocyanins with the quadratic regression equation model established using the Response Surface Methodology (RSM). Result (1) The conditions of the optimal model fit well with actual extraction; (2) The pH differential method was adjusted to a measured wavelength of 530 nm and a solution equilibration time of 80 min; (3) The impact of the four factors of microwave power, material to liquid ratio, methanol concentration and microwave time on the amount of extraction decrease in a progressive order; (4) With the microwave power at 640 W, the methanol concentration as 90%, the microwave time set for 22 min, and the ratio of material (g) to liquid (mL) adjusted as 1∶48, the total anthocyanin extraction in B. striata flower was 6.68 mg·g?1, which was basically consistent with the theoretical prediction value of 6.83 mg·g?1. Conclusion The optimized method can be employed for the extraction and preparation of anthocyanins in B. striata flowers, providing a basis for the comprehensive development and utilization of B. striata flowers. Ch, 8 fig. 2 tab. 22 ref.] |
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