A high-density integrative linkage map for <Emphasis Type="Italic">Gossypium hirsutum</Emphasis> |
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| Authors: | Zhongxu Lin Yanxin Zhang Xianlong Zhang Xiaoping Guo |
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| Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, China |
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| Abstract: | Sequence-related amplified polymorphism (SRAP) combined with SSRs, RAPDs, and RGAPs was used to construct a high density genetic
map for a F2 population derived from the cross DH962 (G. hirsutum accession) × Jimian5 (G. hirsutum cultivar). A total of 4,096 SRAP primer combinations, 6310 SSRs, 600 RAPDs, and 10 RGAPs produced 331, 156, 17 and 2 polymorphic
loci, respectively. Among the 506 loci obtained, 471 loci (309 SRAPs, 144 SSRs, 16 RAPDs and 2 RGAPs) were assigned to 51
linkage groups. Of these, 29 linkage groups were assigned to corresponding chromosomes by SSR markers with known chromosome
locations. The map covered 3070.2 cM with a mean density of 6.5 cM per locus. The segregation distortion in this population
was 9.49%, and these distorted loci tend to cluster at the end of linkage groups or in minor clusters on linkage groups. The
majority of SRAPs in this map provided an effective tool for map construction in G. hirsutum despite of its low polymorphism. This high-density linkage map will be useful for further genetic studies in Upland cotton,
including mapping of loci controlling quantitative traits, and comparative and integrative analysis with other interspecific
and intraspecific linkage maps in cotton. |
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| Keywords: | Cotton Linkage map Sequence-related amplified polymorphisms Simple sequence repeat Resistance gene analog polymorphisms Random amplification polymorphism DNA |
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