| 青海云杉硬枝扦插的激素、年龄和位置效应研究 |
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| 引用本文： | 王军辉,张建国,张守攻.青海云杉硬枝扦插的激素、年龄和位置效应研究[J].西北农林科技大学学报(社会科学版),2006,34(7):65-71. |
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| 作者姓名： | 王军辉 张建国 张守攻 |
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| 作者单位： | [1]中国林业科学研究院林业研究所,北京100091 [2]国家林业局林木培育重点实验室,北京100091 [3]河南农业大学林学园艺学院,河南郑州450001 [4]甘肃天祝县林业局,甘肃天祝733200 |
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| 基金项目： | 国家专项基金;高比容电子铝箔的研究开发与应用项目;退耕还林科技支撑专项基金 |
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| 摘 要： | 为了解青海云杉插穗生根的外在因素和内在生理生化基础,采用网袋容器和自动间歇喷雾扦插繁殖技术,系统研究了青海云杉插穗母株年龄、部位、类型及激素处理等对插穗生根的影响,并采用高效液相色谱法测定了不同插穗类型内源激素吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA3)、玉米素(Z)、玉米素核苷(ZR)、异戊烯基腺嘌呤(IPA)、酚类化合物和糖类的含量。结果表明,以7年生母株枝条作穗条扦插繁殖效果最佳;一级侧枝的生根情况优于二级侧枝和顶枝穗条,树冠下部穗条生根优于上部枝条;激素处理能显著提高硬枝插穗的生根率,其中以IBA 200 m g/kg处理3 h的生根效果最好;邻苯二酸和儿茶酸2种酚类化合物可能对生根有抑制作用;枝条和针叶中三糖含量随母株年龄增加而增大,而葡萄糖含量却呈下降趋势;高含量的Z和ZR均有利于插条生根,IAA对生根无促进作用,IPA对插条生根可能具有一定的抑制作用,单一的某种激素对青海云杉插穗生根无明显影响。
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| 关 键 词： | 青海云杉 硬枝扦插 激素效应 年龄效应 位置效应 |
| 文章编号： | 1671-9387(2006)07-0065-07 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005年9月26日 |
Research of hormone,age and position effect of hardwood cutting in Picea crassifolia Kom |
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| WANG Jun-hui, ZHANG Jian-guo, ZHANG Shou-gong, XU Yang,Yang Hong-qi,ZHANG Jin-xue,SUN Guo-ping.Research of hormone,age and position effect of hardwood cutting in Picea crassifolia Kom[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(7):65-71. |
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| Authors: | WANG Jun-hui ZHANG Jian-guo ZHANG Shou-gong XU Yang Yang Hong-qi ZHANG Jin-xue SUN Guo-ping |
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| Institution: | 1 Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091;China; 2 Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,China; 3 College of Forestry and Horticulture,Henan Agricultural University,Zhengzhou,He’nan 450002,China; 4 Forestry Bureau of Tianzhu County,Tianzhu,Gansu 733200,China) |
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| Abstract: | In order to understand the techniques of cutting propagation and physiological and biochemical basis for hardwood cutting of Picea crassifolia Kom,by cutting method in auto-interval mist system together with some treatments ,the rooting ability of different ortet ages ,different cutting positions ,different branch types, different hormones, some rooting indexes such as rooting ratio, rooting quantity and root length were investigated. The endogenous hormones contents, including IAA, ABA, GA3 and Z, phenol compounds and sugar compound,were measured using HPLC. The result obtained by the experiment indicated that the rooting ratio of seven-year-old ortet was higher than that of twelve-year-old. The rooting effect of cuttings from first-order lateral branches was better than that of cuttings from second -order lateral branches ,and their rooting effect both were better than apical shoots. The rooting ratio of hardwood was increased by plant hormone treatment,especially treated by IBA 250 mg/kg 3 hours. The contents of IAA, ABA,GA3 and Z,phenol compounds and sugar compound as well as the ratio of IAA to ABA,IAA to Z from different cutting types were correlated with the rooting ratio. Hydroxyl benzoate acid and catechol acid perhaps had some restraining functions. Trisaccharide content increased but glucose content decreased with the aging of mother-plants. While the higher-leveled content of Z,ZR contributed to produce more roots,IAA had no any promoting function to cuttings rooting. IPA also can probably control cuttings rooting. Single hormone had no obvious effect on the rooting of hardwood cutting of Picea crassifolia. |
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| Keywords: | Picea crassifolia Kom hardwood cutting hormone effect age effect position effect |
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