| 应用斑点酶联免疫吸附试验快速检测猪弓形虫抗体 |
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| 引用本文： | 童文德,孙连贵,沈柏青,杨福林,董跃,王志泉.应用斑点酶联免疫吸附试验快速检测猪弓形虫抗体[J].中国兽医学报,1989(1). |
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| 作者姓名： | 童文德 孙连贵 沈柏青 杨福林 董跃 王志泉 |
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| 作者单位： | 沈阳军区军马防治研究所
(童文德，孙连贵，沈柏青，杨福林，董跃)，沈阳军区军马防治研究所(王志泉) |
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| 摘 要： | 将斑点酶联免疫吸附试验(Dot—ELISA)用于检测猪弓形虫抗体,并与常规ELISA和IHA法进行了比较。结果,对102份滴度下降的猪阳性血清检测,弓形虫抗体阳性检出率,Dot—ELISA为66.67％(68/102),常规ELISA为48.04％(49/102),IHA为27.45％(28/102);对675头商品猪血清检测,弓形虫抗体阳性检出率,Dot—ELISA为48.15％(325/675),常规ELISA为41.93％(283/675),IHA为33.80％(228/675);与3种寄生虫(猪囊虫、猪旋毛虫、住肉孢子虫)阳性血清无交叉反应;对123份弓形虫抗体阳性和158份阴性猪血清进行3次重复性试验,结果完全一致。结果证明,该法敏感性高,特异性强,操作简便快速(于接到病料后2h报告结果),便于在基层推广使用。
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| 关 键 词： | 斑点酶联免疫吸附试验 弓形虫抗体 检测 |
Rapid Detection of Antibody against Toxoplasma in Pigs With Dot-ELISA |
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| Tong Wende The Military Veterinary Institute in Shenyang Military Area.Rapid Detection of Antibody against Toxoplasma in Pigs With Dot-ELISA[J].Chinese Journal of Veterinary Science,1989(1). |
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| Authors: | Tong Wende The Military Veterinary Institute in Shenyang Military Area |
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| Institution: | Tong Wende The Military Veterinary Institute in Shenyang Military Area |
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| Abstract: | Dot-ELISA was applied for the detection of antibody againstToxoplasma in pigs and a comparison between it and the other methods(ELISAand IHA)was made. Examination of 102 serum samples with low-titer ofspecific antibody from pigs with those methods exhibited that the poisitiverates of Dot-ELISA, ELISA and IHA were 66.67%, 48.04% and 27.45 %,respectively. Of 675 serum samples collected from slaughtered pigs, 325(48. 15%) ,283 (41. 93%) and 228(33. 80%)were diagnosed as poisitive withDot-ELISA, ELISA and IHA respectively. There were no cross reactionwith the known poisitive sera against the other parasites (Cysticercuscellulosae, Trichinella spiralis, Sarcocystis miescheriana). |
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| Keywords: | Dot-ELISA toxoplasma antibody detection |
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