| 发光酶基因(luxAB)标记的荧光假单胞菌CP1108的定殖能力研究 |
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| 引用本文： | 魏永涛,唐欣昀.发光酶基因(luxAB)标记的荧光假单胞菌CP1108的定殖能力研究[J].中国土壤与肥料,2011(6):72-75. |
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| 作者姓名： | 魏永涛 唐欣昀 |
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| 作者单位： | 安徽农业大学生命科学学院,安徽合肥,230036 |
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| 基金项目： | 安徽省科技厅自然基金项目 |
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| 摘 要： | 利用三亲本杂交方法,将luxAB发光酶基因标记至荧光假单胞菌(Pseudomonas fluorescens)CP1108上,研究标记菌株CP1108L的个体生态学特征及其在棉花根圈的定殖动态.结果表明,标记菌株连续传代20次均未发生质粒丢失现象,标记质粒在受体菌株中较为稳定,CP1108L菌株的生长及部分生理生化特性...
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| 关 键 词： | 解磷微生物 荧光假单胞菌 luxAB基因 三亲本杂交 盆栽试验 |
| 收稿时间： | 2011/3/14 0:00:00 |
| 修稿时间： | 2011/5/11 0:00:00 |
Colonization ability of luxAB gene-marked Pseudomonas fluorescens CP1108 in cotton rhizosphere |
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| WEI Yong tao,TANG Xin yun.Colonization ability of luxAB gene-marked Pseudomonas fluorescens CP1108 in cotton rhizosphere[J].Soil and Fertilizer Sciences in China,2011(6):72-75. |
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| Authors: | WEI Yong tao TANG Xin yun |
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| Institution: | WEI Yong-tao,TANG Xin-yun(College of Life Science,Anhui Agricultural University,Hefei 230036) |
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| Abstract: | By the method of tri-parent conjugation,the luxAB gene was introduced into P.fluorescens CP1108.This study aimed at the autecology of P.fluorescens CP1108L and its colonization ability in cotton rhizosphere.The results showed that the marker gene luxAB was successfully transferred into strain CP1108,and the plasmid with luxAB did not lose after 20 generations.Plasmid transformed had little effect on the growth and physiological and biochemical character of strain CP1108L.Strain CP1108L successfully colonize... |
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| Keywords: | phosphate-solubilizing microorganisms Pseudomonas fluorescens luxAB gene tri-parent conjugation pot experiment |
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