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云南超高产橡胶树的SSR、AFLP和TRAP分析
引用本文:张燕燕,黄华孙,胡彦师,程汉,安泽伟.云南超高产橡胶树的SSR、AFLP和TRAP分析[J].热带作物学报,2010,31(6):881-886.
作者姓名:张燕燕  黄华孙  胡彦师  程汉  安泽伟
作者单位:1. 国家橡胶树育种中心,中国热带农业科学院橡胶研究所,海南儋州,571737;海南大学农学院,海南海口,570208
2. 国家橡胶树育种中心,中国热带农业科学院橡胶研究所,海南儋州,571737
基金项目:国家科技支撑计划项目,国家自然科学基金,中央级公益性科研院所基本科研业务费专项基金 
摘    要:利用SSR、AFLP和TRAP3种分子标记对云南勐腊农场发现的2株超高产橡胶树云PR107、云RRIM501与正常产量单株(PR107、RRIM501)进行遗传分析,共筛选到具有多态性的SSR引物7对、AFLP引物28对和TRAP引物12对,3种标记分别检测到多态性条带9、94和21条,平均每对引物可分别检测到1.16、3.36和1.4条多态性条带,结果表明,2株超高产橡胶树的遗传组成均发生了改变。

关 键 词:橡胶树  SSR  AFLP  TRAP  高产

Analysis of Yunnan Super High-yielding Rubber Tree Based on SSR , AFLP and TRAP Markers
Zhang Yanyan,Huang Huasun,Hu Yanshi,Cheng Han and An Zewei.Analysis of Yunnan Super High-yielding Rubber Tree Based on SSR , AFLP and TRAP Markers[J].Chinese Journal of Tropical Crops,2010,31(6):881-886.
Authors:Zhang Yanyan  Huang Huasun  Hu Yanshi  Cheng Han and An Zewei
Abstract:Yield is one of the most important traits and breeding objectives of rubber tree. Two super high-yielding rubber trees, Yun PR107 and Yun RRIM501, were collected from Meng La Farm in Yunnan Province. The super high-yielding rubber tree and normal-yielding rubber tree were investigated with SSR, AFLP and TRAP markers. A total of 7 SSR primers, 28 AFLP primers and 12 TRAP primers produced 7, 92 and 20 polymorphic bands, respectively. Each pair of primers produced 1.16, 3.36 and 1.4 polymorphic bands respectively. The results showed that there were some differences in the genomes between the two super high-yielding rubber trees and normal-yielding rubber tree.
Keywords:SSR  AFLP  TRAP
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