| 兔抗鹅IgG酶标抗体的制备及初步应用 |
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| 引用本文： | 刘菲,唐卫杰,韩清林,赵婷婷,高丽芹,严晓玲.兔抗鹅IgG酶标抗体的制备及初步应用[J].中国畜牧兽医,2011,38(6):91-94. |
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| 作者姓名： | 刘菲 唐卫杰 韩清林 赵婷婷 高丽芹 严晓玲 |
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| 作者单位： | 1. 四川农业大学动物医学院,四川雅安 625014;2. 动物疫病与人类健康四川省重点实验室,四川雅安 625014;3. 四川省大英县畜牧局,四川大英 629300 |
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| 基金项目： | 四川省教育厅自然科学基金重点项目，教育部"长江学者和创新团队发展计划"创新团队项目，现代农业产业技术体系建设专项资金资助项目 |
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| 摘 要： | 为了研究兔抗鹅IgG酶标抗体的制备,试验采用水稀释-辛酸-硫酸铵法粗提,透析除盐,DEAEA-50纤维素层析相结合的方法提取鹅卵黄IgG,经核酸蛋白检测仪和SDS-PAGE测定IgG浓度;制备兔抗鹅IgG酶标抗体;利用酶标抗体检测小鹅瘟病毒(GPV)VP3基因疫苗的免疫效果。结果表明,粗提的IgG浓度为10 mg/mL,抗体纯度可达到94.5%;免疫扩散得出兔抗鹅 IgG抗血清效价约 1∶32,抗体酶标后效价为1∶3000;酶标抗体可以作为检测疫苗效果一种很好的工具。
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| 关 键 词： | 鹅卵黄IgG 提取 酶标 小鹅瘟VP3基因疫苗 |
Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application |
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| LIU Fei,TANG Wei-jie,HAN Qing-lin,ZHAO Ting-ting,GAO Li-qin,YAN Xiao-ling.Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application[J].China Animal Husbandry & Veterinary Medicine,2011,38(6):91-94. |
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| Authors: | LIU Fei TANG Wei-jie HAN Qing-lin ZHAO Ting-ting GAO Li-qin YAN Xiao-ling |
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| Institution: | 1. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China;2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014,China;3. Bureau of Animal Husbandry of Daying, Sichuan Province, Daying 629300, China |
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| Abstract: | To study on preparation of rabbit anti-goose IgG-HRP, the experimentation isolated IgG from the goose egg yolk, and the method was employed to dilute with water-n-octylic acid-ammonia sulfate initial extraction, out salt dialysis and DEAEA-50 chromatography. Protein nucleic acid detector and SDS-PAGE indicated the IgG concentration; the purified IgG was labeled with HRP; effect of GPV VP3 gene vaccine was detected with the labeled antibody. The results indicated that the initial extraction was 10 mg/mL and the purity was 94.5%; the antiserum characterized by immunodiffusion showed that the titer of the antiserum was 1∶32 and specificity of the antiserum was high. The titer of labeled antibody was 1∶3000; the labeled antibody was a good tool to detect the effect of GPV VP3 gene vaccine. |
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| Keywords: | goose egg yolk IgG isolation enzyme labeling pcDNA-VP3 DNA vaccine |
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