| 东北林蛙烂趾病病原菌的分离鉴定及药敏试验 |
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| 引用本文： | 张建平,肖向红,柴龙会.东北林蛙烂趾病病原菌的分离鉴定及药敏试验[J].黑龙江畜牧兽医,2014(1):93-95. |
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| 作者姓名： | 张建平 肖向红 柴龙会 |
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| 作者单位： | 东北林业大学,哈尔滨150040 |
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| 基金项目： | 国家自然科学基金项目（30870301）;黑龙江省自然科学基金重点项目（ZJN0604-02） |
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| 摘 要： | 对东北林蛙烂趾病病原菌进行分离鉴定，并对其药物敏感性进行研究。结果表明：从东北林蛙患病趾处分离到1株溶血性致病茵，经16SrDNA序列分析法鉴定为嗜水气单胞菌。通过对23种抗生素的药敏试验表明，此菌对氟喹诺酮类（如环丙沙星、培氟沙星、诺氟沙星），部分头孢类抗生素（如头孢呋辛、头孢噻肟、头孢吡肟），β-内酰胺类抗生素（如亚胺培南、氨曲南）高度敏感；对部分头孢类抗生素（如头孢噻吩、头孢西丁、头孢他啶），氨基糖苷类抗生素（如卡那霉素、庆大霉素、链霉素）中度敏感；对青霉素类如（青霉素、苯唑西林、氨苄西林、阿莫西林）有耐药性。说明可以考虑用氟喹诺酮类、头孢类或β-内酰胺类抗生素防治东北林蛙烂趾病。
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| 关 键 词： | 东北林蛙 烂趾病 嗜水气单胞菌 药敏试验 |
Isolation and identification of pathogenic bacteria from Rana dybowskii with toe rot disease and the drug sensitivity testing |
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| Institution: | ZHANG Jian - ping, XIAO Xiang - hong, CHAI Long - hui (Northeast Forestry University, Harbin 150040, China) |
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| Abstract: | The pathogenic bacteria was isolated and identified from Rana dybowskii with toe rot disease, and then the drug sensitivity testing was carried out. The results showed that a hemolytic pathogen was isolated from the affected toes of Rana dybowskii with toe rot disease ; it was iden- tified as Aeromonas hydrophila by 16S rDNA sequence analysis. The drug susceptibility testing for 23 kinds of antibiotics showed that the Aero- monas hydrophila was highly sensitive to fluoroquinolones （such as ciprofloxacin, pefloxacin,and norfloxacin）, some cephalosporins （such as cefuroxime, cefotaxime, and cefepime） and β-lactam antibiotics （such as imipenem, and aztreonam） ; moderately sensitive to some cephalo- sporins （such as cephalothin, cefoxitin,and ceftazidime） and aminoglycoside antibiotics （such as kanamycin prime, gentamicin, and strepto- mycin） ; resistance to penicillin （such as penicillin, oxacillin, ampicillin, and amoxicillin）. The results indicate that fluoroquinolones, cepha- losporins or β-lactam antibiotics may be considered to prevent the toe rot disease in Rana dybowskii. |
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| Keywords: | Rana dybowskii toe rot disease Aeromonas hydrophila sensitivity test |
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