| 甘蓝亲本防退化技术研究 |
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| 引用本文： | 汪承刚,王素.甘蓝亲本防退化技术研究[J].安徽农业科学,2009,37(12):5370-5371. |
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| 作者姓名： | 汪承刚 王素 |
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| 作者单位： | 安徽省淮南市农业科学研究所,安徽淮南,232008;安徽省淮南市农业科学研究所,安徽淮南,232008 |
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| 摘 要： | 目的]探索甘蓝亲本防退化技术。方法]以“春魁”甘蓝父母本为材料，选取培养10d后的无菌苗茎尖为诱导材料镑种在培养基上进行诱导培养、愈伤组织继代培养、芽分化培养、生根培养和炼苗移栽，研究采用茎尖组培快繁技术繁殖甘蓝原种。结果]诱导出的不定芽在2种分化培养基内幼苗长势均良好，在MS＋6-BA2．0mg／L＋NAA0．2mg／L培养基中幼苗增殖倍数为3—5倍，且生成大量根系：在MS＋6-BA2．0mg／L培养基中幼苗增殖倍数可达到5～7倍，没有根系生成。2个亲本利用组培繁殖植株的种子产量高于常规繁殖植株的产量，9701—2和HT502亲本组培繁育的种子产量分别比常规繁殖的高16．8％和39．1％。结论]采用茎尖组培快繁技术繁殖甘蓝原种，可减少甘蓝原原种用量，提高繁殖系数和种子发芽率。
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| 关 键 词： | 茎尖 组培 原种 繁殖系数 发芽率 |
Study on the Technique of Preventing Cabbage Parents from Degradation |
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| WANG Cheng-gang et al.Study on the Technique of Preventing Cabbage Parents from Degradation[J].Journal of Anhui Agricultural Sciences,2009,37(12):5370-5371. |
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| Authors: | WANG Cheng-gang |
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| Institution: | WANG Cheng-gang et al(Huainan Institute of Agricultural Science,Huainan,Anhui 232008) |
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| Abstract: | Objective]The aim was to discuss technique of preventing cabbage parents from degradation.Method] With Chunkui parents of cabbage as the material,the shoot tip of aseptic seedling after culturing for 10d was selected as the induced material,which was inoculated on the medium to make the induction culture,callus subculture,bud differentiation culture,rooting culture and acclimatization and transplants.Reproducing the cabbage stock by using the tissue culture and rapid propagation technique of stem tip was ... |
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| Keywords: | Stem tip Tissue culture Stock seed Propagation coefficient Germination rate |
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