| 体外法研究不同淀粉与滤纸纤维比例日粮对瘤胃发酵和微生物的影响 |
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| 引用本文： | 王梦芝,王洪荣,李国祥,张洁,曹恒春.体外法研究不同淀粉与滤纸纤维比例日粮对瘤胃发酵和微生物的影响[J].动物营养学报,2007,19(6):654-662. |
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| 作者姓名： | 王梦芝 王洪荣 李国祥 张洁 曹恒春 |
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| 作者单位： | 扬州大学动物科学与技术学院,扬州,225009 |
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| 基金项目： | National Natural Science Foundation of China(No:30571344) |
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| 摘 要： | 以3只瘘管山羊作为瘤胃液供体,用体外法研究不同底物对瘤胃发酵及瘤胃微生物的影响。底物设计:可溶性淀粉/滤纸纤维(NSC/SC):100∶0,70∶30,50∶50,30∶70,0∶100。结果表明:在NSC/SC的比例为30∶70组微生物产量和纤维素降解率最高,瘤胃液中的NH3-N浓度最低,发酵状态最佳;原虫与细菌的比例,总体上有随NSC/SC下降先成上升后降低的趋势,在NSC/SC的比例为50∶50组达到最高峰。细菌16srRNA的V3可变区PCR-SSCP各组图谱反映了细菌类群内部种属因底物的改变而发生了应变。原虫显微观察结果表明随NSC/SC下降,内毛属与等毛虫原虫的比例下降,而双毛属与头毛亚科原虫的比例增加,即原虫类群内部种属也对底物变化的产生应变。不同淀粉与纤维素比例水平底物不仅影响了瘤胃发酵也影响了瘤胃微生物的群系特征。动物营养学报,2007,19(6):654-662]
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| 关 键 词： | 可溶性淀粉/滤纸纤维 瘤胃发酵 瘤胃微生物 PCR-SSCP 细胞计数 |
| 修稿时间： | 2007年2月2日 |
Effects of Rations in Different Starch to Filter Paper Ratio on Rumen Fermentation and Microbes in Vitro |
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| WANG Meng-zhi,WANG Hong-rong,LI Guo-xiang,ZHANG Jie,CAO Heng-chun.Effects of Rations in Different Starch to Filter Paper Ratio on Rumen Fermentation and Microbes in Vitro[J].Acta Zoonutrimenta Sinica,2007,19(6):654-662. |
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| Authors: | WANG Meng-zhi WANG Hong-rong LI Guo-xiang ZHANG Jie CAO Heng-chun |
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| Abstract: | Three goats fisted with cannulas were used to investigate the effects of rations with different starch to filter paper ratio on rumen fermentation and microbes in vitro. The dietary ratio of starch to filter paper (NSC/SC)was designed as follows: 100:0, 70:30, 50:50, 30:70 and 0:100. The results showed that cellulose degradability and microbial biomass were the highest when NSC/SC ratio in the culture was set to 30:70; it was further observed when NSC/SC ratio decreased, the index of protozoa to bacteria ratio (P/B) had a tendency of increase firstly, followed by decline P/B ratio was the highest in the group whose NSC/SC ratio in the culture was 50:50;PCR-SSCP analysis of V3 in bacteria 16s rRNA showed that the profile of bacteria was subjected to substrates. Additionally, cell-counting results showed that, the percentages of Entodinium spp. and Isotricha spp. decreased,whereas percentages of Diplodinium spp. and Ophryoscolecinae increased with the decrease of NSC/SC ratio,which also revealed that, the profile of protozoa was subjected to substrates too. In conclusion, dietary compositioninfluenced both rumen fermentation and microbial characteristics. |
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| Keywords: | Starch to filter paper ratio Rumen fermentation Rumen microbes PCR-SSCP Cell-counting |
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