| 甜菜夜蛾颗粒体病毒毒力的生物测定 |
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| 引用本文： | 林国宪,黄志鹏,关雄.甜菜夜蛾颗粒体病毒毒力的生物测定[J].福建农林大学学报(自然科学版),1995(3). |
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| 作者姓名： | 林国宪 黄志鹏 关雄 |
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| 作者单位： | 福建农业大学生物中心 |
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| 摘 要： | 以甜菜夜蛾２—３龄幼虫为供试虫，对国内外首次发现的甜菜夜蛾颗粒体病毒（Ｌａｐｈｙｇｍａｅｘｉｇｕｅｇｒａｎｙｌｏｓｉｓｖｉｒｕｓ，ＬｅＧＶ）进行了生物测定．在２３—２５℃相对湿度７０％—８０％下其样品死亡机率值与浓度对数值的回归方程是ｙ＝０．４５ｘ＋３．７６（ｒ＝０．９９），ＬＣ５０＝５７２．４颗粒体·ｍＬ－１，ＬＣ５０的９５％置信区为１５８—２０８４颗粒体·ｍＬ－１．甜菜夜蛾不同龄期幼虫对ＬｅＧＶ的敏感性不同，初孵幼虫敏感性最强，２、３龄次之，４龄幼虫极不敏感．此外，贮藏期温度对ＬｅＧＶ感染力的影响也进行了初步研究．
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| 关 键 词： | 甜菜夜蛾颗粒体病毒 毒力 生物测定 |
Bioassay on infectivity of Laphygma exigue Granulosis Virus |
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| Lin Guoxian,Huang Zhipen,Guan Xiong.Bioassay on infectivity of Laphygma exigue Granulosis Virus[J].Journal of Fujian Agricultural and Forestry University,1995(3). |
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| Authors: | Lin Guoxian Huang Zhipen Guan Xiong |
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| Abstract: | Bioassay on the granulos virus of Laphygma exigue (LeGV) was conducted by using the 2nd-3rd instar larvae of L. exigue as test insect.Under the conditionsof temperature 23-25℃ and relative humidity 70%- 80%,the regression equation for the value of sample death probabilty and concentration logarithmic valuewas y=0.45x+3.76 (r =0.99),its LC50 572.4 capsules·mL-1 with 95% fiducial linits 158-2 084 capsules· mL-1.The susceptibility to LeGV was different among various instar larvaes,it turned out that the first instar larvae were most susceptible,and the following sequences were the 2nd,3rd and 4th instar.Moreover,the effect of storage temperatura on the infectivity of LeGV was also studied. |
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| Keywords: | Laphygma exigue granylosis virus (LeGV) infectivity bioassay |
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