| 琥珀酰辅酶A转移酶硝基化酪氨酸位点特异单克隆抗体的制备 |
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| 引用本文： | 何靖玄,黄丽华,黄妤,张学文.琥珀酰辅酶A转移酶硝基化酪氨酸位点特异单克隆抗体的制备[J].湖南农业大学学报(自然科学版),2011,37(4):436-439. |
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| 作者姓名： | 何靖玄 黄丽华 黄妤 张学文 |
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| 作者单位： | 湖南农业大学生物科学技术学院,湖南长沙,410128 |
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| 基金项目： | 湖南省研究上科研创新项日 |
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| 摘 要： | 线粒体琥珀酰辅酶A转移酶(SCOT)含有4个酪氨酸残基,其4、76位点酪氨酸(Tyr4、Tyr76)的硝基化可导致其酶活性下降,制备特定的抗体可对其硝基化水平和位点进行检测.应用淋巴细胞杂交瘤技术,以化学合成的含琥珀酰辅酶A转移酶硝基化Tyr4和Tyr76肽段为半抗原,偶联钥孔血蓝蛋白(KLH)后,作为免疫原免疫Bal...
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| 关 键 词： | 线粒体琥珀酰辅酶A转移酶 硝基化酪氨酸 单克隆抗体 |
Development of monoclonal antibody against nitrated Tyr4 and Tyr76 in succinyl CoA: 3-0xoacid CoA transferase |
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| HE Jing-xuan,HUANG Li-hua,HUANG Yu,ZHANG Xue-wen.Development of monoclonal antibody against nitrated Tyr4 and Tyr76 in succinyl CoA: 3-0xoacid CoA transferase[J].Journal of Hunan Agricultural University,2011,37(4):436-439. |
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| Authors: | HE Jing-xuan HUANG Li-hua HUANG Yu ZHANG Xue-wen |
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| Institution: | HE Jing-xuan,HUANG Li-hua,HUANG Yu,ZHANG Xue-wen(College of Bioscience and Biotechology,Hunan Agricultural University,Changsha 410128,China) |
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| Abstract: | There are 4 tyrosine residues in succinyl CoA: 3-oxoacid CoA transferase(SCOT),in which nitration of Tyr4 and Tyr76 will lead to change of the structure and decrease of enzymatic activity.In this study artificial synthetic peptides covering the SCOT Tyr4 and Tyr76 region with the tyrosine nitrated were employed as hapten and conjugated to keyhole limpet hemocyanin(KLH) to immunize Balb/c mouse.The monoclonal antibodies against nitrated Tyr4 and Tyr76 in SCOT are developed.After screening of massive cell lin... |
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| Keywords: | succinyl CoA: 3-oxoacid CoA transferase nitrotyrosine monoclonal antibody |
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