| 农杆菌介导的蓝色基因转化中国水仙 |
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| 引用本文： | 戴艺民,林江波,王伟英,邹晖,吴水金,林一心.农杆菌介导的蓝色基因转化中国水仙[J].农业生物技术学报,2010,18(2):231-238. |
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| 作者姓名： | 戴艺民 林江波 王伟英 邹晖 吴水金 林一心 |
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| 作者单位： | 福建省农科院甘蔗研究所,漳州,363005 |
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| 基金项目： | 福建省自然科学基金，福建省农科院科技创新重点项目 |
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| 摘 要： | 从矮牵牛(Petunia hybrida)花瓣中分离得到编码蓝色基因F3'5'H(类黄酮3',5'羟基化酶)的Hf2基因(1527 bp),从牵牛(Ipomoea nil)花瓣中分离得到dfr基因(二氢黄酮醇4-还原酶,1212 bp),将正向Hf2片段和反向dfr片段连接到pBRLys双元载体质粒上,得到具有Ubi(ubiquitin)组成型启动子的植物表达载体pU-bi-Hf2-dfr.利用冻融法将双价重组质粒pUbi-Hf2一afr导入农杆菌(Agrobacterium tumefaciens)菌株EHA105中,菌落PCR鉴定农杆菌转化子.以中国水仙(Narcissus tazetta var.chinensis)愈伤组织为材料,进行遗传转化.CTAB法提取130株中国水仙遗传转化再生苗的基因组DNA,进行PCR和PCR-Southem blot检测,获得3株阳性转基因植株.
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| 关 键 词： | 蓝色基因 中国水仙 遗传转化 |
Transformation of Blue Gene in Narcissus tazetta var.chinensis Mediated by Agrobacterium tumefaciens |
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| Dai Yimin,Lin Jiangbo,Wang Weiying,Zou Hui,Wu Shuijin,Lin Yixin.Transformation of Blue Gene in Narcissus tazetta var.chinensis Mediated by Agrobacterium tumefaciens[J].Journal of Agricultural Biotechnology,2010,18(2):231-238. |
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| Authors: | Dai Yimin Lin Jiangbo Wang Weiying Zou Hui Wu Shuijin Lin Yixin |
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| Institution: | Dai Yimin Lin Jiangbo Wang Weiying Zou Hui Wu Shuijin Lin Yixin Sugarcane Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,363005 |
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| Abstract: | Hf2 gene(1 527 bp) encoding flavonoid 3',5'-hydroxylase(F3'5'H) and dihydroflavonol 4-reductase(dfr)(1 212 bp) gene were isolated from Petunia hybrida and Ipomoea nil petals respectively.Hf2 and anti sense dfr genes were subcloned into pBRLys vector together generating pUbi-Hf2-dfr vector which contained Ubiquitin(Ubi) promoter, and then the pUbi-Hf2-dfr was transferred into Agrobacterium tumefaciens strain EHA105.Result of PCR amplification with transformant colony showed that the expression vector pUbi-Hf... |
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| Keywords: | Blue gene Narcissus tazetta var Chinensis Genetic transformation |
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