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不同种植密度下半夏叶片气孔特性的变化初探
引用本文:李祥栋,黄梅,张明生,李立青,高晓峰,刘贵贤.不同种植密度下半夏叶片气孔特性的变化初探[J].安徽农业科学,2014(25):8554-8555,8562.
作者姓名:李祥栋  黄梅  张明生  李立青  高晓峰  刘贵贤
作者单位:贵州大学生命科学学院,贵州贵阳,550025;贵州省赫章县林业局,贵州赫章,553200
基金项目:贵州省中药现代化科技产业研究开发专项(黔科合中药字[2011]5065号);中央财政林业科技推广示范资金([2012]TQ08号);贵州省中药材现代产业技术体系建设项目(GZ-CYTX-02).
摘    要:目的]探索不同种植密度下半夏叶片气孔特性的变化。方法]取5个不同种植密度下的半夏叶片,撕取其下表皮制片、显微观察,随机选取5个视野,记载气孔频度、气孔长轴和短轴。结果]不同种植密度下半夏叶片气孔频度的差异不显著,其变化范围为80.48~92.09个/mm2;而随着种植密度的增加,叶片气孔器的长轴和短轴长度均表现出"上升-平稳-上升"的变化趋势。当半夏块茎用量从1 875 kg/hm2增加至3 375 kg/hm2时,气孔器的长轴长度从41.943 0μm增加至50.941 4μm,短轴长度从25.739 3μm增加至35.342 8μm,且最高值和最低值差异极显著。结论]种植密度对半夏叶片气孔器的大小影响较为明显。

关 键 词:半夏  种植密度  气孔器  气孔频度

Preliminary Study on Stomatal Characteristics of Pinellia ternata in Different Planting Density
Institution:LI Xiang-dong,HUANG Mei ZHANG Ming-sheng(School of Life Sciences, Guizhou University, Guiyang,Guizhou 550025)
Abstract:Objective] The research aimed to investigate the stomatal characteristics changes of Pinellia ternate leaves in different planting densities.Method] To pick the leaves of Pinellia ternate under five different planting density,to tear lower epidermis of the leaves and to make the temporary slides.In microscopic observation,the selected randomly five views were used to record the stomatal frequency,long axis and short axis.Result] The difference of stomatal frequency in leaves under different planting densities was not significant,its changc range was from 80.48 to 92.09 per square millimeter.The "up-steady-up" change trends of major axis length and minor axis length of stomatal apparatus were appeared with the increase of planting density.The length axis length was increased from 41.943 0 μm to 50.941 4 μn,and minor axis length was increased from 25.739 3 μm to 35.342 8 μm with the tuber dosage was increased from 1 875 kg/hm2 to 3 375 kg/hm2,and the difference between maximum value and the minimum value was highly significant.Conclusion] The influence that planting density in regard to the stomata1 apparatus of Pinellia ternate leaves was significant.
Keywords:Pinellia ternata (Thunb  ) Breit    Planting density  Stomatal apparatus  Stomatal frequency
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