| 饥饿胁迫下大鳞副泥鳅外周红细胞的形态变化 |
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| 引用本文： | 杜,良,钟爱华,周兵飞等.饥饿胁迫下大鳞副泥鳅外周红细胞的形态变化[J].安徽农业科学,2014(23):7761-7763,7803. |
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| 作者姓名： | 杜 良 钟爱华 周兵飞等 |
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| 作者单位： | 浙江海洋学院水产学院,浙江舟山316004 |
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| 基金项目： | 浙江海洋学院大学生科技创新项目（浙海院团[2013]38号）;浙江省教育厅科研项目（Y201328411）. |
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| 摘 要： | 目的]为研究饥饿胁迫下鱼类的生理生化变化提供参考。方法]采用传统瑞氏染色法研究饥饿胁迫下大鳞副泥鳅外周红细胞的形态变化。结果]大鳞副泥鳅的成熟红细胞逐渐发生变化,先出现核膜溶解,然后出现细胞形变,接着出现核质扩散和细胞膜溶解,最后整个成熟红细胞膜完全溶解,核染色反应由紫黑色变为紫红色最后变为粉红色。大鳞副泥鳅的幼稚红细胞则明显增多。结论]在饥饿胁迫下大鳞副泥鳅外周红细胞出现核异常,主要表现为核膜溶解和核质扩散,因此红细胞核异常可以作为饥饿胁迫的指标。
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| 关 键 词： | 饥饿胁迫 大鳞副泥鳅 红细胞 形态 |
Morphological Changes of Peripheral Erythrocyte in Paramisgurnus dabryanus under Starvation Stress |
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| Institution: | DU Liang, ZHONG Ai-hua et al (Fishery School of Zhejiang Ocean University, Zhoushan, Zhejiang 316022) |
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| Abstract: | Objective] The research aimed to provide references for studying the physiological and biochemical changes of fishes under star- vation stress. Method] Using traditional Wrightg staining method, the morphology changes of peripheral erythrocyte in Paramisgurnus dabry-anus under starvation stress were studied. Result] The morphology of mature erythrocyte in P. dabryanus gradually changed. First, the nu- clear membrane was dissolved, then the cell deformation occurred and the nucleoplasm diffused and cytomembrane began to dissolve, finally the membrane of mature erythrocyte completely dissolved. The staining reaction of nuclear was from purple-black to purple-red, and finally turned to pink. The The number of immature erythrocyte in P. dabryanus was significantly increased. Conclusion] The nuclear anomalies of peripheral erythrocyte in P. dabryanus under starvation stress occurred, and their manifestations were the dissolution of karyolemma and diffu-sion of karvoDlasms. Therefore the nuclear anomalies of ervthrocvte could be used as the index of starvation stress. |
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| Keywords: | Starvation Paramisgurnus dabryanus Erythrocyte Morphology |
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