| 水稻纹枯病拮抗细菌的筛选·鉴定及其活性成分的发酵制备 |
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| 引用本文： | 黄河,鄢凯舟,林元山,丰来,罗志威.水稻纹枯病拮抗细菌的筛选·鉴定及其活性成分的发酵制备[J].安徽农业科学,2014(25):8584-8586,8647. |
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| 作者姓名： | 黄河 鄢凯舟 林元山 丰来 罗志威 |
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| 作者单位： | 湖南农业大学东方科技学院,湖南长沙,410128;湖南泰谷生物科技股份有限公司,湖南长沙,410205 |
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| 基金项目： | 湖南省大学生创新项目（DFCXS201106）;湖南省长沙市现代农业科技资金专项（k1308090-21）. |
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| 摘 要： | 目的]探究水稻纹枯病拮抗细菌的抑菌活性成分的发酵制备条件。方法]通过点接种于平板与水稻纹枯病对峙生长对水稻纹枯病拮抗细菌进行了初筛,通过摇瓶发酵进行了复筛,并通过形态鉴定及16S RNA测序构建系统发育树对所得菌株进行鉴定;通过单因素试验研究了所得菌株的抑菌活性成分的发酵制备条件。结果]初筛获得17株水稻纹枯病的拮抗细菌,复筛获得菌株Lys-67,该菌株的发酵液对水稻纹枯病生长具有明显的抑制作用,菌株Lys-67鉴定为枯草芽孢杆菌(Bacillus subtilis)。经单因素试验,菌株Lys-67利用麸皮5.0%,于35℃、摇床转速200 r/min条件下发酵3 d,经牛津杯打孔法检测,150μl发酵液中活性成分抑制水稻纹枯病的抑菌圈达36 mm。结论]为菌株Lys-67的进一步研究及生防应用奠定了基础。
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| 关 键 词： | 水稻纹枯病 拮抗菌 筛选 发酵 生物防治 |
Screening,Identification of Antagonistic Bacteria against Rhizoctonia solani and Preparation of Its Active Ingredient by Fermentation |
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| Institution: | HUANG He,YAN Kai-zhou,LIN Yuan-shan(Oriental Science and Technology College of Hunan Agricultural University, Changsha, Hunan 410128) |
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| Abstract: | Objective] The aim was to study the fermentation conditions of antagonistic bacteria active ingredient against Rhizoctonia solani.Method] Some antagonistic bacteria against R.solani were screened through point inoculating contrary to R.solani on agar petri plates and shaking flask fermentation; the obtained strain was identified through morphological identification and 16S RNA sequencing to construct phylogenetic tree ; and fermentation conditions of active ingredient of the obtained strain against R.solani were studied through single factor experiment.Result] Seventeen strains of bacteria antagonist R.solani were obtained,and strain Lys-67 was obtained finally; strain Lys-67 was identified as Bacillus subtilis.Also,150 μl fermentation broth of strain Lys-67 resulted in 36 mm of inhibition zone against R.solani,determining by method of drilling hole with Oxford cup on agar plate,while strain Lys-67 was cultured in wheat bran 5.0％,at 35 C,rotation speed 200 r/min for three days.Conclusion] The results lay basis for further study and application of strain Lys-67. |
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| Keywords: | Rhizoctonia solani Antagonistic bacteria Screening Fermentation Biological control |
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