| 响应面法优化变种米曲霉产木聚糖酶发酵培养基 |
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| 引用本文： | 王中兴.响应面法优化变种米曲霉产木聚糖酶发酵培养基[J].农产品加工．学刊,2012(5):39-42. |
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| 作者姓名： | 王中兴 |
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| 作者单位： | 安康学院农学与生命科学院,陕西安康,725000 |
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| 基金项目： | 安康学院高层次人才项目 |
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| 摘 要： | 采用响应面法对木聚糖酶产生菌变种米曲霉(Aspergillus oryzae)RM-503的发酵培养基进行了优化。用Plackett-Burman法(Plackett-Burman Design,P-B)对影响变种米曲霉产木聚糖酶发酵的相关因素进行了评价。筛选有显著效应的2个因素:KH2PO4和ZnSO4.7H2O。在此基础上,采用最陡爬坡路径逼近最大响应区域,利用中心旋转组合设计及响应面分析确定影响木聚糖酶产量的关键因素KH2PO4和ZnSO4.7H2O的最佳水平。经过上述优化,木聚糖酶产量比基本培养条件下提高了1.399倍,达到8.681 U/mL。
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| 关 键 词： | 木聚糖酶 米曲霉 Plackett-Burman设计 响应面法 优化 |
Medium Optimization of Aspergillus oryzae var for Xylanase Using Response Surface Methodology |
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| WANG Zhong-xing.Medium Optimization of Aspergillus oryzae var for Xylanase Using Response Surface Methodology[J].Nongchanpin Jlagong.Xuekan,2012(5):39-42. |
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| Authors: | WANG Zhong-xing |
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| Institution: | WANG Zhong-xing(Collegeof Agricultureand LifeSciences,AnkangUniversity,Ankang,Shaanxi 725000,China) |
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| Abstract: | The fermentation medium of Aspergillus oryzae RM-503 producing xylanase is optimized by using response surface methodology(RSM).In the first step of optimization,with Plackett-Burman design,KH 2 PO 4 and ZnSO 4 ·7H 2 O are found to be the important factors affecting xylanase production significantly.Then the steepest ascent experiment is adopted to approach the optimal region of the medium composition.At last,the central composition design and response surface methodology are used to determine the optimal levels of the main factors.After optimization,the yield of xylanase production is increased by 139.9% compared with that in the original medium. |
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| Keywords: | xylanase Aspergillus oryzae Plackett-Burman design response surface methodology optimization |
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